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10.1038/s41589-020-0542-9 http://scihub22266oqcxt.onion/10.1038/s41589-020-0542-9 32424303!7388658!32424303     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=32424303&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Chem+Biol 2020 ; 16 (8): 826-833 Nephropedia Template TP {{tp|p=32424303|t=2020. Multiplexed GTPase and GEF biosensor imaging enables network connectivity analysis |pdf=|usr=}}{{32424303}} gab.com Text Multiplexed GTPase and GEF biosensor imaging enables network connectivity analysis JO: Nat Chem Biol http://www.kidney.de/pget.php?&tw=1&pmid=32424303 #FOAvip Here we generate fluorescence resonance energy transfer biosensors for guanine exchange factors (GEFs) by inserting a fluorescent protein pair in a structural 'hinge' common to many GEFs. Fluorescent biosensors can map the activation of signaling molecules in space and time, but it has not been possible to quantify how different activation events affect one another or contribute to a specific cell behavior. By imaging the GEF biosensors in the same cells as red-shifted biosensors of Rho GTPases, we can apply partial correlation analysis to parse out the extent to which each GEF contributes to the activation of a specific GTPase in regulating cell movement. Through analysis of spontaneous cell protrusion events, we identify when and where the GEF Asef regulates the GTPases Cdc42 and Rac1 to control cell edge dynamics. This approach exemplifies a powerful means to elucidate the real-time connectivity of signal transduction networks. Twit Text FOAVip Multiplexed GTPase and GEF biosensor imaging enables network connectivity analysis ????Nat Chem Biol http://www.kidney.de/pget.php?&tw=1&pmid=32424303 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32424303 #FOAvip English Wikipedia <ref>{{Cite pmid|32424303}}</ref> Multiplexed GTPase and GEF biosensor imaging enables network connectivity analysis #MMPMID32424303 Marston DJ; Vilela M; Huh J; Ren J; Azoitei ML; Glekas G; Danuser G; Sondek J; Hahn KM Nat Chem Biol 2020[Aug]; 16 (8): 826-833 PMID32424303show ga Here we generate fluorescence resonance energy transfer biosensors for guanine exchange factors (GEFs) by inserting a fluorescent protein pair in a structural 'hinge' common to many GEFs. Fluorescent biosensors can map the activation of signaling molecules in space and time, but it has not been possible to quantify how different activation events affect one another or contribute to a specific cell behavior. By imaging the GEF biosensors in the same cells as red-shifted biosensors of Rho GTPases, we can apply partial correlation analysis to parse out the extent to which each GEF contributes to the activation of a specific GTPase in regulating cell movement. Through analysis of spontaneous cell protrusion events, we identify when and where the GEF Asef regulates the GTPases Cdc42 and Rac1 to control cell edge dynamics. This approach exemplifies a powerful means to elucidate the real-time connectivity of signal transduction networks. |Amino Acid Sequence/genetics[MESH] |Biosensing Techniques/methods[MESH] |Fluorescence Resonance Energy Transfer/*methods[MESH] |Guanine Nucleotide Exchange Factors/*metabolism[MESH] |Protein Binding/genetics[MESH] |Sequence Homology, Amino Acid[MESH] |Signal Transduction/genetics[MESH] |cdc42 GTP-Binding Protein/metabolism[MESH] |rac1 GTP-Binding Protein/metabolism[MESH]Multiplexed GTPase and GEF biosensor imaging enables network connectiv(epmc).pdf DeepDyve Pubget Overpricing