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10.1016/j.bbrc.2020.04.136 http://scihub22266oqcxt.onion/10.1016/j.bbrc.2020.04.136 32416961!7190499!32416961     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biochem+Biophys+Res+Commun 2020 ; 527 (3): 618-623 Nephropedia Template TP {{tp|p=32416961|t=2020. Biochemical characterization of SARS-CoV-2 nucleocapsid protein |pdf=|usr=}}{{32416961}} gab.com Text Biochemical characterization of SARS-CoV-2 nucleocapsid protein JO: Biochem Biophys Res Commun http://www.kidney.de/pget.php?&tw=1&pmid=32416961 #FOAvip The nucleocapsid (N) protein is an important antigen for coronavirus, which participate in RNA package and virus particle release. In this study, we expressed the N protein of SARS-CoV-2 and characterized its biochemical properties. Static light scattering, size exclusive chromatography, and small-angle X-ray scattering (SAXS) showed that the purified N protein is largely a dimer in solution. CD spectra showed that it has a high percentage of disordered region at room temperature while it was best structured at 55 degrees C, suggesting its structural dynamics. Fluorescence polarization assay showed it has non-specific nucleic acid binding capability, which raised a concern in using it as a diagnostic marker. Immunoblot assays confirmed the presence of IgA, IgM and IgG antibodies against N antigen in COVID-19 infection patients' sera, proving the importance of this antigen in host immunity and diagnostics. Twit Text FOAVip Biochemical characterization of SARS-CoV-2 nucleocapsid protein ????Biochem Biophys Res Commun http://www.kidney.de/pget.php?&tw=1&pmid=32416961 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32416961 #FOAvip English Wikipedia <ref>{{Cite pmid|32416961}}</ref> Biochemical characterization of SARS-CoV-2 nucleocapsid protein #MMPMID32416961 Zeng W; Liu G; Ma H; Zhao D; Yang Y; Liu M; Mohammed A; Zhao C; Yang Y; Xie J; Ding C; Ma X; Weng J; Gao Y; He H; Jin T Biochem Biophys Res Commun 2020[Jun]; 527 (3): 618-623 PMID32416961show ga The nucleocapsid (N) protein is an important antigen for coronavirus, which participate in RNA package and virus particle release. In this study, we expressed the N protein of SARS-CoV-2 and characterized its biochemical properties. Static light scattering, size exclusive chromatography, and small-angle X-ray scattering (SAXS) showed that the purified N protein is largely a dimer in solution. CD spectra showed that it has a high percentage of disordered region at room temperature while it was best structured at 55 degrees C, suggesting its structural dynamics. Fluorescence polarization assay showed it has non-specific nucleic acid binding capability, which raised a concern in using it as a diagnostic marker. Immunoblot assays confirmed the presence of IgA, IgM and IgG antibodies against N antigen in COVID-19 infection patients' sera, proving the importance of this antigen in host immunity and diagnostics. |Antibodies, Viral/blood[MESH] |Betacoronavirus/*chemistry[MESH] |COVID-19[MESH] |Coronavirus Infections[MESH] |Humans[MESH] |Nucleic Acids[MESH] |Nucleocapsid Proteins/*chemistry[MESH] |Pandemics[MESH] |Pneumonia, Viral[MESH] |Protein Binding[MESH] |Protein Multimerization[MESH] |Protein Structure, Tertiary[MESH] |SARS-CoV-2[MESH] |Scattering, Small Angle[MESH]Biochemical characterization of SARS-CoV-2 nucleocapsid protein (epmc).pdf DeepDyve Pubget Overpricing