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10.1016/j.jcv.2020.104423 http://scihub22266oqcxt.onion/10.1016/j.jcv.2020.104423 32416598!7204723!32416598     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Clin+Virol 2020 ; 128 (ä): 104423 Nephropedia Template TP {{tp|p=32416598|t=2020. SARS-CoV-2 detection by direct rRT-PCR without RNA extraction |pdf=|usr=}}{{32416598}} gab.com Text SARS-CoV-2 detection by direct rRT-PCR without RNA extraction JO: J Clin Virol http://www.kidney.de/pget.php?&tw=1&pmid=32416598 #FOAvip Rapid and reliable screening of SARS-CoV-2 is fundamental to assess viral spread and limit the pandemic we are facing. In this study, we compared direct rRT-PCR method (without RNA extraction) using SeeGene AllplexTM 2019-nCoV rRT-PCR with the RealStar(R) SARS-CoV-2 rRT-PCR kit (Altona Diagnostics). Furthermore, we assessed the impact of swab storage media composition on PCR efficiency. We show that SeeGene and Altona's assays provide similar efficiency. Importantly, we provide evidence that RNA extraction can be successfully bypassed when samples are stored in UTM medium or in molecular water but not when samples are stored in saline solution and in Hanks medium. Twit Text FOAVip SARS-CoV-2 detection by direct rRT-PCR without RNA extraction ????J Clin Virol http://www.kidney.de/pget.php?&tw=1&pmid=32416598 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32416598 #FOAvip English Wikipedia <ref>{{Cite pmid|32416598}}</ref> SARS-CoV-2 detection by direct rRT-PCR without RNA extraction #MMPMID32416598 Merindol N; Pepin G; Marchand C; Rheault M; Peterson C; Poirier A; Houle C; Germain H; Danylo A J Clin Virol 2020[Jul]; 128 (ä): 104423 PMID32416598show ga Rapid and reliable screening of SARS-CoV-2 is fundamental to assess viral spread and limit the pandemic we are facing. In this study, we compared direct rRT-PCR method (without RNA extraction) using SeeGene AllplexTM 2019-nCoV rRT-PCR with the RealStar(R) SARS-CoV-2 rRT-PCR kit (Altona Diagnostics). Furthermore, we assessed the impact of swab storage media composition on PCR efficiency. We show that SeeGene and Altona's assays provide similar efficiency. Importantly, we provide evidence that RNA extraction can be successfully bypassed when samples are stored in UTM medium or in molecular water but not when samples are stored in saline solution and in Hanks medium. |*Specimen Handling[MESH] |Betacoronavirus/genetics/*isolation & purification[MESH] |COVID-19[MESH] |Coronavirus Infections/*diagnosis/virology[MESH] |Humans[MESH] |Nasopharynx/virology[MESH] |Pandemics[MESH] |Pneumonia, Viral/*diagnosis/virology[MESH] |RNA, Viral/genetics[MESH] |Reverse Transcriptase Polymerase Chain Reaction/*methods[MESH]SARS-CoV-2 detection by direct rRT-PCR without RNA extraction (epmc).pdf DeepDyve Pubget Overpricing