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10.3201/eid2608.201246 http://scihub22266oqcxt.onion/10.3201/eid2608.201246 32396505!7392423!32396505     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Emerg+Infect+Dis 2020 ; 26 (8): 1654-65 Nephropedia Template TP {{tp|p=32396505|t=2020. US CDC Real-Time Reverse Transcription PCR Panel for Detection of Severe Acute Respiratory Syndrome Coronavirus 2 |pdf=|usr=}}{{32396505}} gab.com Text US CDC Real-Time Reverse Transcription PCR Panel for Detection of Severe Acute Respiratory Syndrome Coronavirus 2 JO: Emerg Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=32396505 #FOAvip Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was identified as the etiologic agent associated with coronavirus disease, which emerged in late 2019. In response, we developed a diagnostic panel consisting of 3 real-time reverse transcription PCR assays targeting the nucleocapsid gene and evaluated use of these assays for detecting SARS-CoV-2 infection. All assays demonstrated a linear dynamic range of 8 orders of magnitude and an analytical limit of detection of 5 copies/reaction of quantified RNA transcripts and 1 x 10(-1.5) 50% tissue culture infectious dose/mL of cell-cultured SARS-CoV-2. All assays performed comparably with nasopharyngeal and oropharyngeal secretions, serum, and fecal specimens spiked with cultured virus. We obtained no false-positive amplifications with other human coronaviruses or common respiratory pathogens. Results from all 3 assays were highly correlated during clinical specimen testing. On February 4, 2020, the Food and Drug Administration issued an Emergency Use Authorization to enable emergency use of this panel. Twit Text FOAVip US CDC Real-Time Reverse Transcription PCR Panel for Detection of Severe Acute Respiratory Syndrome Coronavirus 2 ????Emerg Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=32396505 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32396505 #FOAvip English Wikipedia <ref>{{Cite pmid|32396505}}</ref> US CDC Real-Time Reverse Transcription PCR Panel for Detection of Severe Acute Respiratory Syndrome Coronavirus 2 #MMPMID32396505 Lu X; Wang L; Sakthivel SK; Whitaker B; Murray J; Kamili S; Lynch B; Malapati L; Burke SA; Harcourt J; Tamin A; Thornburg NJ; Villanueva JM; Lindstrom S Emerg Infect Dis 2020[Aug]; 26 (8): 1654-65 PMID32396505show ga Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was identified as the etiologic agent associated with coronavirus disease, which emerged in late 2019. In response, we developed a diagnostic panel consisting of 3 real-time reverse transcription PCR assays targeting the nucleocapsid gene and evaluated use of these assays for detecting SARS-CoV-2 infection. All assays demonstrated a linear dynamic range of 8 orders of magnitude and an analytical limit of detection of 5 copies/reaction of quantified RNA transcripts and 1 x 10(-1.5) 50% tissue culture infectious dose/mL of cell-cultured SARS-CoV-2. All assays performed comparably with nasopharyngeal and oropharyngeal secretions, serum, and fecal specimens spiked with cultured virus. We obtained no false-positive amplifications with other human coronaviruses or common respiratory pathogens. Results from all 3 assays were highly correlated during clinical specimen testing. On February 4, 2020, the Food and Drug Administration issued an Emergency Use Authorization to enable emergency use of this panel. |Betacoronavirus/*genetics[MESH] |Biomarkers/analysis[MESH] |COVID-19[MESH] |Centers for Disease Control and Prevention, U.S.[MESH] |Coronavirus Infections/*diagnosis/virology[MESH] |Coronavirus Nucleocapsid Proteins[MESH] |DNA Primers/chemical synthesis/genetics[MESH] |Feces/virology[MESH] |Fluoresceins/chemistry[MESH] |Fluorescent Dyes/chemistry[MESH] |Humans[MESH] |Limit of Detection[MESH] |Nasopharynx/virology[MESH] |Nucleocapsid Proteins/*genetics[MESH] |Pandemics[MESH] |Phosphoproteins[MESH] |Pneumonia, Viral/*diagnosis/virology[MESH] |RNA, Viral/*genetics[MESH] |Real-Time Polymerase Chain Reaction/*methods/standards[MESH] |Reproducibility of Results[MESH] |SARS-CoV-2[MESH] |Sputum/virology[MESH]US CDC Real-Time Reverse Transcription PCR Panel for Detection of Seve(epmc).pdf DeepDyve Pubget Overpricing