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10.1016/j.antiviral.2020.104819 http://scihub22266oqcxt.onion/10.1016/j.antiviral.2020.104819 32389733!7205714!32389733     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Antiviral+Res 2020 ; 179 (ä): 104819 Nephropedia Template TP {{tp|p=32389733|t=2020. Identification of potassium and calcium channel inhibitors as modulators of polyomavirus endosomal trafficking |pdf=|usr=}}{{32389733}} gab.com Text Identification of potassium and calcium channel inhibitors as modulators of polyomavirus endosomal trafficking JO: Antiviral Res http://www.kidney.de/pget.php?&tw=1&pmid=32389733 #FOAvip During virus entry, members of the Polyomaviridae transit the endolysosomal network en route to the endoplasmic reticulum (ER), from which degraded capsids escape into the cytoplasm and enter the nucleus. Emerging evidence suggests that viruses require both endosomal acidification and the correct ionic balance of K(+) and Ca(2+) ions in endosomes for correct virus trafficking and genome release. Here, using two polyomaviruses with different capsid architectures, namely Simian virus 40 (SV40) and Merkel cell polyomavirus (MCPyV), we describe methods to rapidly quantify virus infection using IncuCyte ZOOM imaging analysis, and use this system to investigate the role of both K(+) and Ca(2+) channels during the early stages of virus entry. Using broad spectrum blockers of both K(+) and Ca(2+) channels to specifically target host cell ion channel functionality, we show that MCPyV, but not SV40 can be inhibited by K(+) channel modulators, whilst both viruses are restricted by the broad spectrum Ca(2+) channel inhibitor verapamil. Using a panel of more specific Ca(2+) blockers, we show that both MCPyV and SV40 are dependent on the activity of two-pore Ca(2+) channels (TPCs), as the TPC-specific blocker tetrandrine prevented capsid disassembly and nuclear transport required for virus entry. We therefore reveal a novel target to restrict the entry of polyomaviruses, which given the known role of TPCs during endolysosomal-ER fusion, is likely to be applicable to other viruses that transit this pathway. Twit Text FOAVip Identification of potassium and calcium channel inhibitors as modulators of polyomavirus endosomal trafficking ????Antiviral Res http://www.kidney.de/pget.php?&tw=1&pmid=32389733 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32389733 #FOAvip English Wikipedia <ref>{{Cite pmid|32389733}}</ref> Identification of potassium and calcium channel inhibitors as modulators of polyomavirus endosomal trafficking #MMPMID32389733 Dobson SJ; Mankouri J; Whitehouse A Antiviral Res 2020[Jul]; 179 (ä): 104819 PMID32389733show ga During virus entry, members of the Polyomaviridae transit the endolysosomal network en route to the endoplasmic reticulum (ER), from which degraded capsids escape into the cytoplasm and enter the nucleus. Emerging evidence suggests that viruses require both endosomal acidification and the correct ionic balance of K(+) and Ca(2+) ions in endosomes for correct virus trafficking and genome release. Here, using two polyomaviruses with different capsid architectures, namely Simian virus 40 (SV40) and Merkel cell polyomavirus (MCPyV), we describe methods to rapidly quantify virus infection using IncuCyte ZOOM imaging analysis, and use this system to investigate the role of both K(+) and Ca(2+) channels during the early stages of virus entry. Using broad spectrum blockers of both K(+) and Ca(2+) channels to specifically target host cell ion channel functionality, we show that MCPyV, but not SV40 can be inhibited by K(+) channel modulators, whilst both viruses are restricted by the broad spectrum Ca(2+) channel inhibitor verapamil. Using a panel of more specific Ca(2+) blockers, we show that both MCPyV and SV40 are dependent on the activity of two-pore Ca(2+) channels (TPCs), as the TPC-specific blocker tetrandrine prevented capsid disassembly and nuclear transport required for virus entry. We therefore reveal a novel target to restrict the entry of polyomaviruses, which given the known role of TPCs during endolysosomal-ER fusion, is likely to be applicable to other viruses that transit this pathway. |Animals[MESH] |Benzylisoquinolines/pharmacology[MESH] |Calcium Channel Blockers/*pharmacology[MESH] |Cell Line[MESH] |Cell Movement[MESH] |Chlorocebus aethiops[MESH] |Drug Discovery[MESH] |Endosomes/*physiology/virology[MESH] |HEK293 Cells[MESH] |Humans[MESH] |Merkel cell polyomavirus/drug effects/physiology[MESH] |Polyomavirus/*drug effects/physiology[MESH] |Potassium Channel Blockers/*pharmacology[MESH] |Simian virus 40/drug effects/physiology[MESH] |Verapamil/pharmacology[MESH] |Vero Cells[MESH]Identification of potassium and calcium channel inhibitors as modulato(epmc).pdf DeepDyve Pubget Overpricing