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10.1093/infdis/jiaa243 http://scihub22266oqcxt.onion/10.1093/infdis/jiaa243 32382737!7239108!32382737     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Infect+Dis 2020 ; 222 (2): 189-193 Nephropedia Template TP {{tp|p=32382737|t=2020. A Peptide-Based Magnetic Chemiluminescence Enzyme Immunoassay for Serological Diagnosis of Coronavirus Disease 2019 |pdf=|usr=}}{{32382737}} gab.com Text A Peptide-Based Magnetic Chemiluminescence Enzyme Immunoassay for Serological Diagnosis of Coronavirus Disease 2019 JO: J Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=32382737 #FOAvip BACKGROUND: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a novel beta-coronavirus, causes severe pneumonia and has spread throughout the globe rapidly. The disease associated with SARS-CoV-2 infection is named coronavirus disease 2019 (COVID-19). To date, real-time reverse-transcription polymerase chain reaction (RT-PCR) is the only test able to confirm this infection. However, the accuracy of RT-PCR depends on several factors; variations in these factors might significantly lower the sensitivity of detection. METHODS: In this study, we developed a peptide-based luminescent immunoassay that detected immunoglobulin (Ig)G and IgM. The assay cutoff value was determined by evaluating the sera from healthy and infected patients for pathogens other than SARS-CoV-2. RESULTS: To evaluate assay performance, we detected IgG and IgM in the sera from confirmed patients. The positive rate of IgG and IgM was 71.4% and 57.2%, respectively. CONCLUSIONS: Therefore, combining our immunoassay with real-time RT-PCR might enhance the diagnostic accuracy of COVID-19. Twit Text FOAVip A Peptide-Based Magnetic Chemiluminescence Enzyme Immunoassay for Serological Diagnosis of Coronavirus Disease 2019 ????J Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=32382737 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32382737 #FOAvip English Wikipedia <ref>{{Cite pmid|32382737}}</ref> A Peptide-Based Magnetic Chemiluminescence Enzyme Immunoassay for Serological Diagnosis of Coronavirus Disease 2019 #MMPMID32382737 Cai XF; Chen J; Li Hu J; Long QX; Deng HJ; Liu P; Fan K; Liao P; Liu BZ; Wu GC; Chen YK; Li ZJ; Wang K; Zhang XL; Tian WG; Xiang JL; Du HX; Wang J; Hu Y; Tang N; Lin Y; Ren JH; Huang LY; Wei J; Gan CY; Chen YM; Gao QZ; Chen AM; He CL; Wang DX; Hu P; Zhou FC; Huang AL; Wang DQ J Infect Dis 2020[Jun]; 222 (2): 189-193 PMID32382737show ga BACKGROUND: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a novel beta-coronavirus, causes severe pneumonia and has spread throughout the globe rapidly. The disease associated with SARS-CoV-2 infection is named coronavirus disease 2019 (COVID-19). To date, real-time reverse-transcription polymerase chain reaction (RT-PCR) is the only test able to confirm this infection. However, the accuracy of RT-PCR depends on several factors; variations in these factors might significantly lower the sensitivity of detection. METHODS: In this study, we developed a peptide-based luminescent immunoassay that detected immunoglobulin (Ig)G and IgM. The assay cutoff value was determined by evaluating the sera from healthy and infected patients for pathogens other than SARS-CoV-2. RESULTS: To evaluate assay performance, we detected IgG and IgM in the sera from confirmed patients. The positive rate of IgG and IgM was 71.4% and 57.2%, respectively. CONCLUSIONS: Therefore, combining our immunoassay with real-time RT-PCR might enhance the diagnostic accuracy of COVID-19. |Adult[MESH] |Antibodies, Viral/*blood[MESH] |Betacoronavirus/*immunology[MESH] |COVID-19[MESH] |COVID-19 Testing[MESH] |COVID-19 Vaccines[MESH] |Clinical Laboratory Techniques/*methods[MESH] |Coronavirus Infections/*diagnosis/immunology[MESH] |Female[MESH] |Humans[MESH] |Immunoenzyme Techniques/*methods[MESH] |Immunoglobulin G/blood[MESH] |Immunoglobulin M/blood[MESH] |Luminescent Measurements[MESH] |Male[MESH] |Middle Aged[MESH] |Pandemics[MESH] |Peptides/immunology[MESH] |Pneumonia, Viral/*diagnosis/immunology[MESH] |Real-Time Polymerase Chain Reaction[MESH] |Reverse Transcriptase Polymerase Chain Reaction[MESH] |SARS-CoV-2[MESH] |Sensitivity and Specificity[MESH] |Serologic Tests/*methods[MESH]A Peptide-Based Magnetic Chemiluminescence Enzyme Immunoassay for Sero(epmc).pdf DeepDyve Pubget Overpricing