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10.1128/JCM.00890-20 http://scihub22266oqcxt.onion/10.1128/JCM.00890-20 32381643!7383551!32381643     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=32381643&cmd=llinks): Failed to open stream: HTTP request failed! 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Comparison of Two High-Throughput Reverse Transcription-PCR Systems for the Detection of Severe Acute Respiratory Syndrome Coronavirus 2 |pdf=|usr=}}{{32381643}} gab.com Text Comparison of Two High-Throughput Reverse Transcription-PCR Systems for the Detection of Severe Acute Respiratory Syndrome Coronavirus 2 JO: J Clin Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=32381643 #FOAvip Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has emerged as the cause of a worldwide pandemic. Many commercial SARS-CoV-2 reverse transcription-PCR (RT-PCR) assays have received Emergency Use Authorization from the U.S. Food and Drug Administration. However, there are limited data describing their performance, in particular the performance of high-throughput SARS-CoV-2 RT-PCR systems. We analyzed the diagnostic performance of two high-throughput systems: cobas 6800 and Panther Fusion, and their associated RT-PCR assays, with a collection of 389 nasopharyngeal specimens. The overall agreement between the platforms was 96.4% (375/389). Cohen's kappa analysis rated the strength of agreement between the two platforms as "almost perfect" (kappa = 0.922; standard error, 0.051). Furthermore, there was no significant difference between corresponding cycle threshold values generated on the two systems (P value = 0.88; Student's t test). Taken together, these data imply that the two platforms can be considered comparable in terms of their clinical performance. We believe that this information will be useful for those who have already adopted these platforms or are seeking to implement high-throughput RT-PCR testing to stem the SARS-CoV-2 pandemic. Twit Text FOAVip Comparison of Two High-Throughput Reverse Transcription-PCR Systems for the Detection of Severe Acute Respiratory Syndrome Coronavirus 2 ????J Clin Microbiol http://www.kidney.de/pget.php?&tw=1&pmid=32381643 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32381643 #FOAvip English Wikipedia <ref>{{Cite pmid|32381643}}</ref> Comparison of Two High-Throughput Reverse Transcription-PCR Systems for the Detection of Severe Acute Respiratory Syndrome Coronavirus 2 #MMPMID32381643 Craney AR; Velu PD; Satlin MJ; Fauntleroy KA; Callan K; Robertson A; La Spina M; Lei B; Chen A; Alston T; Rozman A; Loda M; Rennert H; Cushing M; Westblade LF J Clin Microbiol 2020[Jul]; 58 (8): ä PMID32381643show ga Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has emerged as the cause of a worldwide pandemic. Many commercial SARS-CoV-2 reverse transcription-PCR (RT-PCR) assays have received Emergency Use Authorization from the U.S. Food and Drug Administration. However, there are limited data describing their performance, in particular the performance of high-throughput SARS-CoV-2 RT-PCR systems. We analyzed the diagnostic performance of two high-throughput systems: cobas 6800 and Panther Fusion, and their associated RT-PCR assays, with a collection of 389 nasopharyngeal specimens. The overall agreement between the platforms was 96.4% (375/389). Cohen's kappa analysis rated the strength of agreement between the two platforms as "almost perfect" (kappa = 0.922; standard error, 0.051). Furthermore, there was no significant difference between corresponding cycle threshold values generated on the two systems (P value = 0.88; Student's t test). Taken together, these data imply that the two platforms can be considered comparable in terms of their clinical performance. We believe that this information will be useful for those who have already adopted these platforms or are seeking to implement high-throughput RT-PCR testing to stem the SARS-CoV-2 pandemic. |*High-Throughput Screening Assays[MESH] |Betacoronavirus/genetics/*isolation & purification[MESH] |COVID-19[MESH] |Coronavirus Infections/*diagnosis/virology[MESH] |Humans[MESH] |Nasopharynx/virology[MESH] |Pandemics[MESH] |Pneumonia, Viral/*diagnosis/virology[MESH] |Reverse Transcriptase Polymerase Chain Reaction/*methods[MESH] |SARS-CoV-2[MESH]Comparison of Two High-Throughput Reverse Transcription-PCR Systems fo(epmc).pdf DeepDyve Pubget Overpricing