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10.1016/j.virusres.2020.197988 http://scihub22266oqcxt.onion/10.1016/j.virusres.2020.197988 32380210!7198166!32380210     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Virus+Res 2020 ; 285 (ä): 197988 Nephropedia Template TP {{tp|p=32380210|t=2020. The impacts of viral inactivating methods on quantitative RT-PCR for COVID-19 |pdf=|usr=}}{{32380210}} gab.com Text The impacts of viral inactivating methods on quantitative RT-PCR for COVID-19 JO: Virus Res http://www.kidney.de/pget.php?&tw=1&pmid=32380210 #FOAvip OBJECTIVE: This paper aims to explore the effect of four virus inactivation methods on the rapid detection results of COVID-19 nucleic acid. METHODS: Collected samples of nasopharyngeal swabs from 2 patients diagnosed with COVID-19 at the First People's Hospital of Zhaoqing City, each of sample was divided into 5 groups (groupA approximately E): A:Non-inactivated raw sample; B:75 % ethanol inactivation; C:56 ? incubation for 30?min inactivation; D:65 ? incubation for 10?min inactivation; E:Pre-inactivation using RNA virus special preservation fluid added into the sampling tube to treated the nasopharyngeal swab sample separately, using real-time fluorescent RT-PCR to detect the N gene of COVID-19 and the ORF1ab gene simultaneously. All the groups are diluted in 1:2, 1:4, 1:8 ratios. The objectives are to compare the effect of the varied inactivation method on CT(Cycle Threshold)results in PCR, conduct correlation and Bland-Altman analysis. RESULTS: For the N gene and ORF1ab gene, the CT values of 4 inactivated and Non-inactivated treatment were correlated (P?0.001). The results of the four treatment methods and specimens without inactivated treatment have shown good consistency. CONCLUSION: The treatment of nasopharyngeal swab specimens using mentioned four inactivated methods had no significant effect on the subsequent detection of the new COVID-19 nucleic acid test. Lab test-persons can flexibly adopt pre-inactivation methods to ensure the accuracy of virus nucleic acid test results, meanwhile guarantee the safety of lab test-persons. Twit Text FOAVip The impacts of viral inactivating methods on quantitative RT-PCR for COVID-19 ????Virus Res http://www.kidney.de/pget.php?&tw=1&pmid=32380210 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32380210 #FOAvip English Wikipedia <ref>{{Cite pmid|32380210}}</ref> The impacts of viral inactivating methods on quantitative RT-PCR for COVID-19 #MMPMID32380210 Wang Y; Song W; Zhao Z; Chen P; Liu J; Li C Virus Res 2020[Aug]; 285 (ä): 197988 PMID32380210show ga OBJECTIVE: This paper aims to explore the effect of four virus inactivation methods on the rapid detection results of COVID-19 nucleic acid. METHODS: Collected samples of nasopharyngeal swabs from 2 patients diagnosed with COVID-19 at the First People's Hospital of Zhaoqing City, each of sample was divided into 5 groups (groupA approximately E): A:Non-inactivated raw sample; B:75 % ethanol inactivation; C:56 ? incubation for 30?min inactivation; D:65 ? incubation for 10?min inactivation; E:Pre-inactivation using RNA virus special preservation fluid added into the sampling tube to treated the nasopharyngeal swab sample separately, using real-time fluorescent RT-PCR to detect the N gene of COVID-19 and the ORF1ab gene simultaneously. All the groups are diluted in 1:2, 1:4, 1:8 ratios. The objectives are to compare the effect of the varied inactivation method on CT(Cycle Threshold)results in PCR, conduct correlation and Bland-Altman analysis. RESULTS: For the N gene and ORF1ab gene, the CT values of 4 inactivated and Non-inactivated treatment were correlated (P?0.001). The results of the four treatment methods and specimens without inactivated treatment have shown good consistency. CONCLUSION: The treatment of nasopharyngeal swab specimens using mentioned four inactivated methods had no significant effect on the subsequent detection of the new COVID-19 nucleic acid test. Lab test-persons can flexibly adopt pre-inactivation methods to ensure the accuracy of virus nucleic acid test results, meanwhile guarantee the safety of lab test-persons. |*Betacoronavirus/genetics/physiology[MESH] |*Reverse Transcriptase Polymerase Chain Reaction[MESH] |*Virus Inactivation[MESH] |COVID-19[MESH] |COVID-19 Testing[MESH] |COVID-19 Vaccines[MESH] |Clinical Laboratory Techniques/*methods[MESH] |Coronavirus Infections/*diagnosis[MESH] |Coronavirus Nucleocapsid Proteins[MESH] |Ethanol[MESH] |Hot Temperature[MESH] |Humans[MESH] |Nasopharynx/virology[MESH] |Nucleocapsid Proteins/analysis/genetics[MESH] |Pandemics[MESH] |Phosphoproteins[MESH] |Pneumonia, Viral/*diagnosis[MESH] |Polyproteins[MESH] |Preservation, Biological[MESH] |RNA, Viral/analysis[MESH] |SARS-CoV-2[MESH] |Specimen Handling/methods[MESH]The impacts of viral inactivating methods on quantitative RT-PCR for C(epmc).pdf DeepDyve Pubget Overpricing