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10.1172/jci.insight.139042 http://scihub22266oqcxt.onion/10.1172/jci.insight.139042 32379723!7406253!32379723     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=32379723&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       JCI+Insight 2020 ; 5 (12): ä Nephropedia Template TP {{tp|p=32379723|t=2020. Molecular detection of SARS-CoV-2 in formalin-fixed, paraffin-embedded specimens |pdf=|usr=}}{{32379723}} gab.com Text Molecular detection of SARS-CoV-2 in formalin-fixed, paraffin-embedded specimens JO: JCI Insight http://www.kidney.de/pget.php?&tw=1&pmid=32379723 #FOAvip Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the cause of human coronavirus disease 2019 (COVID-19), emerged in Wuhan, China, in December 2019. The virus rapidly spread globally, resulting in a public health crisis including almost 5 million cases and 323,256 deaths as of May 21, 2020. Here, we describe the identification and evaluation of commercially available reagents and assays for the molecular detection of SARS-CoV-2 in infected FFPE cell pellets. We identified a suitable rabbit polyclonal anti-SARS-CoV spike protein antibody and a mouse monoclonal anti-SARS-CoV nucleocapsid protein (NP) antibody for cross-detection of the respective SARS-CoV-2 proteins by IHC and immunofluorescence assay (IFA). Next, we established RNAscope in situ hybridization (ISH) to detect SARS-CoV-2 RNA. Furthermore, we established a multiplex FISH (mFISH) to detect positive-sense SARS-CoV-2 RNA and negative-sense SARS-CoV-2 RNA (a replicative intermediate indicating viral replication). Finally, we developed a dual staining assay using IHC and ISH to detect SARS-CoV-2 antigen and RNA in the same FFPE section. It is hoped that these reagents and assays will accelerate COVID-19 pathogenesis studies in humans and in COVID-19 animal models. Twit Text FOAVip Molecular detection of SARS-CoV-2 in formalin-fixed, paraffin-embedded specimens ????JCI Insight http://www.kidney.de/pget.php?&tw=1&pmid=32379723 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32379723 #FOAvip English Wikipedia <ref>{{Cite pmid|32379723}}</ref> Molecular detection of SARS-CoV-2 in formalin-fixed, paraffin-embedded specimens #MMPMID32379723 Liu J; Babka AM; Kearney BJ; Radoshitzky SR; Kuhn JH; Zeng X JCI Insight 2020[Jun]; 5 (12): ä PMID32379723show ga Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the cause of human coronavirus disease 2019 (COVID-19), emerged in Wuhan, China, in December 2019. The virus rapidly spread globally, resulting in a public health crisis including almost 5 million cases and 323,256 deaths as of May 21, 2020. Here, we describe the identification and evaluation of commercially available reagents and assays for the molecular detection of SARS-CoV-2 in infected FFPE cell pellets. We identified a suitable rabbit polyclonal anti-SARS-CoV spike protein antibody and a mouse monoclonal anti-SARS-CoV nucleocapsid protein (NP) antibody for cross-detection of the respective SARS-CoV-2 proteins by IHC and immunofluorescence assay (IFA). Next, we established RNAscope in situ hybridization (ISH) to detect SARS-CoV-2 RNA. Furthermore, we established a multiplex FISH (mFISH) to detect positive-sense SARS-CoV-2 RNA and negative-sense SARS-CoV-2 RNA (a replicative intermediate indicating viral replication). Finally, we developed a dual staining assay using IHC and ISH to detect SARS-CoV-2 antigen and RNA in the same FFPE section. It is hoped that these reagents and assays will accelerate COVID-19 pathogenesis studies in humans and in COVID-19 animal models. |Animals[MESH] |Antibodies, Viral/immunology[MESH] |Antigens, Viral/isolation & purification[MESH] |Betacoronavirus/genetics/immunology/*isolation & purification[MESH] |COVID-19[MESH] |COVID-19 Testing[MESH] |Clinical Laboratory Techniques/methods[MESH] |Coronavirus Infections/diagnosis/pathology/*virology[MESH] |Formaldehyde[MESH] |Humans[MESH] |Immunohistochemistry[MESH] |In Situ Hybridization[MESH] |Mice[MESH] |Nucleocapsid Proteins/immunology[MESH] |Pandemics[MESH] |Paraffin Embedding/methods[MESH] |Pathology, Molecular/methods[MESH] |Pneumonia, Viral/pathology/*virology[MESH] |RNA, Viral/isolation & purification[MESH] |Rabbits[MESH] |SARS-CoV-2[MESH]Molecular detection of SARS-CoV-2 in formalin-fixed, paraffin-embedded(epmc).pdf DeepDyve Pubget Overpricing