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10.1128/mSphere.00199-20

http://scihub22266oqcxt.onion/10.1128/mSphere.00199-20
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suck abstract from ncbi


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pmid32376700      mSphere 2020 ; 5 (3): ä
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  • Detecting and Monitoring Porcine Hemagglutinating Encephalomyelitis Virus, an Underresearched Betacoronavirus #MMPMID32376700
  • Mora-Diaz JC; Magtoto R; Houston E; Baum D; Carrillo-Avila JA; Temeeyasen G; Zimmerman J; Pineyro P; Gimenez-Lirola L
  • mSphere 2020[May]; 5 (3): ä PMID32376700show ga
  • Members of family Coronaviridae cause a variety of diseases in birds and mammals. Porcine hemagglutinating encephalomyelitis virus (PHEV), a lesser-researched coronavirus, can infect naive pigs of any age, but clinical disease is observed in pigs /=0.6 was both sensitive and specific, i.e., all PHEV-inoculated pigs were seropositive from days postinoculation 10 to 42, and no cross-reactivity was observed in samples from other groups. The PHEV S1 ELISA was then used to estimate PHEV seroprevalence in U.S. sow herds (19 states) using 2,756 serum samples from breeding females (>28 weeks old) on commercial farms (n = 104) with no history of PHEV-associated disease. The overall seroprevalence was 53.35% (confidence interval [CI], +/-1.86%) and herd seroprevalence was 96.15% (CI, +/-3.70%).IMPORTANCE There is a paucity of information concerning the ecology of porcine hemagglutinating encephalomyelitis virus (PHEV) in commercial swine herds. This study provided evidence that PHEV infection is endemic and highly prevalent in U.S. swine herds. These results raised questions for future studies regarding the impact of endemic PHEV on swine health and the mechanisms by which this virus circulates in endemically infected populations. Regardless, the availability of the validated PHEV S1 enzyme-linked immunosorbent assay (ELISA) provides the means for swine producers to detect and monitor PHEV infections, confirm prior exposure to the virus, and to evaluate the immune status of breeding herds.
  • |Animals[MESH]
  • |Antibodies, Viral/*blood/immunology[MESH]
  • |Betacoronavirus 1/immunology/*isolation & purification[MESH]
  • |Coronavirus Infections/diagnosis/*epidemiology/*veterinary[MESH]
  • |Cross Reactions/immunology[MESH]
  • |Enzyme-Linked Immunosorbent Assay/methods[MESH]
  • |Immunoglobulin G/blood/immunology[MESH]
  • |Porcine Respiratory Coronavirus/immunology[MESH]
  • |Porcine epidemic diarrhea virus/immunology[MESH]
  • |Seroepidemiologic Studies[MESH]
  • |Swine[MESH]
  • |Swine Diseases/diagnosis/*epidemiology[MESH]
  • |Transmissible gastroenteritis virus/immunology[MESH]


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