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10.12122/j.issn.1673-4254.2020.03.03 http://scihub22266oqcxt.onion/10.12122/j.issn.1673-4254.2020.03.03 32376571!7167315!32376571     free     free     free Warning: Undefined variable $yww in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 538 Warning: Undefined variable $yww in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 538       Nan+Fang+Yi+Ke+Da+Xue+Xue+Bao 2020 ; 40 (3): 316-320 Nephropedia Template TP {{tp|p=32376571|t=2020. Effect of heat inactivation of blood samples on the efficacy of three detection methods of SARS-CoV-2 antibodies |pdf=|usr=}}{{32376571}} gab.com Text Effect of heat inactivation of blood samples on the efficacy of three detection methods of SARS-CoV-2 antibodies JO: Nan Fang Yi Ke Da Xue Xue Bao http://www.kidney.de/pget.php?&tw=1&pmid=32376571 #FOAvip OBJECTIVE: To evaluate the effects of heat inactivation of blood samples at 56? for 30 min on the results of SARS-CoV-2 antibody detection using different methods. METHODS: This retrospective study was conducted in 11 patients with established diagnosis of COVID-19 and 10 patients with diseases other than COVID- 19 in our hospital. We collected samples of serum, plasma and whole blood from each patient between February, 12 and 18, 2020, and with a double- blind design, the samples were examined for SARS-CoV-2 antibodies before and after heat inactivation at 56 ? for 30 min. In all the samples, the total SARS-CoV-2 antibodies were detected using immunochromatography, and the IgM antibodies were detected using fluorescence immunochromatography; the IgM and IgG antibodies in the serum and plasma samples detected with chemiluminescence immunoassay. We compared the detection results and analyzed the correlation of semi-quantitative detection results of IgM and IgG antibodies before and after heat inactivation of the samples. RESULTS: With immuno-chromatography, the coincidence rate of the total SARS-CoV-2 antibody detection before and after heat inactivation of the serum and plasma samples was 90.0% in COVID-19 cases and 100.0% in the negative cases, resulting in a total coincidence rate 95.2%; for the whole blood samples, the total coincidence rates of the total SARS-CoV-2 antibodies were 100.0%. For detection of IgM antibodies in the serum, plasma and whole blood samples using fluorescence immunochromatography, the coincidence rates in SARS-CoV-2-positive and negative cases and the total coincidence rate before and after inactivation were 100.0%, 0 and 47.6%, respectively. For detection of serum IgM and IgG antibodies and plasma IgG antibodies with chemiluminescence immunoassay, the coincidence rates in SARS-CoV-2-positive and negative cases and the total coincidence rate before and after inactivation were all 100.0%, and the total coincidence rate of plasma IgM antibodies was 95.2%. Pearson correlation analysis of the semi-quantitative results of IgM and IgG detection in the serum and plasma samples showed a correlation coefficient of 0.9999 (95%CI: 0.9998-1.000, P < 0.001) between the results before and after sample inactivation. CONCLUSIONS: Heat inactivation of blood samples at 56 ? for 30 min does not obviously affect the results of immunochromatography and chemiluminescent immunoassay for detection of SARS-COV-2 antibodies but can reduce the risk of infection for the operators. Heat-inactivated samples can not be used in fluorescence immunochromatography for SARS-CoV-2 antibody detection. Twit Text FOAVip Effect of heat inactivation of blood samples on the efficacy of three detection methods of SARS-CoV-2 antibodies ????Nan Fang Yi Ke Da Xue Xue Bao http://www.kidney.de/pget.php?&tw=1&pmid=32376571 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32376571 #FOAvip English Wikipedia <ref>{{Cite pmid|32376571}}</ref> Effect of heat inactivation of blood samples on the efficacy of three detection methods of SARS-CoV-2 antibodies #MMPMID32376571 Xue X; Zhu C; Huang S; Pan L; Xu J; Li W Nan Fang Yi Ke Da Xue Xue Bao 2020[Mar]; 40 (3): 316-320 PMID32376571show ga OBJECTIVE: To evaluate the effects of heat inactivation of blood samples at 56? for 30 min on the results of SARS-CoV-2 antibody detection using different methods. METHODS: This retrospective study was conducted in 11 patients with established diagnosis of COVID-19 and 10 patients with diseases other than COVID- 19 in our hospital. We collected samples of serum, plasma and whole blood from each patient between February, 12 and 18, 2020, and with a double- blind design, the samples were examined for SARS-CoV-2 antibodies before and after heat inactivation at 56 ? for 30 min. In all the samples, the total SARS-CoV-2 antibodies were detected using immunochromatography, and the IgM antibodies were detected using fluorescence immunochromatography; the IgM and IgG antibodies in the serum and plasma samples detected with chemiluminescence immunoassay. We compared the detection results and analyzed the correlation of semi-quantitative detection results of IgM and IgG antibodies before and after heat inactivation of the samples. RESULTS: With immuno-chromatography, the coincidence rate of the total SARS-CoV-2 antibody detection before and after heat inactivation of the serum and plasma samples was 90.0% in COVID-19 cases and 100.0% in the negative cases, resulting in a total coincidence rate 95.2%; for the whole blood samples, the total coincidence rates of the total SARS-CoV-2 antibodies were 100.0%. For detection of IgM antibodies in the serum, plasma and whole blood samples using fluorescence immunochromatography, the coincidence rates in SARS-CoV-2-positive and negative cases and the total coincidence rate before and after inactivation were 100.0%, 0 and 47.6%, respectively. For detection of serum IgM and IgG antibodies and plasma IgG antibodies with chemiluminescence immunoassay, the coincidence rates in SARS-CoV-2-positive and negative cases and the total coincidence rate before and after inactivation were all 100.0%, and the total coincidence rate of plasma IgM antibodies was 95.2%. Pearson correlation analysis of the semi-quantitative results of IgM and IgG detection in the serum and plasma samples showed a correlation coefficient of 0.9999 (95%CI: 0.9998-1.000, P < 0.001) between the results before and after sample inactivation. CONCLUSIONS: Heat inactivation of blood samples at 56 ? for 30 min does not obviously affect the results of immunochromatography and chemiluminescent immunoassay for detection of SARS-COV-2 antibodies but can reduce the risk of infection for the operators. Heat-inactivated samples can not be used in fluorescence immunochromatography for SARS-CoV-2 antibody detection. |*Betacoronavirus[MESH] |Antibodies, Viral[MESH] |COVID-19[MESH] |Coronavirus Infections/*diagnosis[MESH] |Enzyme-Linked Immunosorbent Assay[MESH] |Hot Temperature[MESH] |Humans[MESH] |Immunoglobulin G[MESH] |Immunoglobulin M[MESH] |Pandemics[MESH] |Pneumonia, Viral/*diagnosis[MESH] |Retrospective Studies[MESH] Effect of heat inactivation of blood samples on the efficacy of three(epmc).pdf DeepDyve Pubget Overpricing