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10.1681/ASN.2020040432 http://scihub22266oqcxt.onion/10.1681/ASN.2020040432 32371536!7460898!32371536     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Am+Soc+Nephrol 2020 ; 31 (8): 1683-1687 Nephropedia Template TP {{tp|p=32371536|t=2020. Ultrastructural Evidence for Direct Renal Infection with SARS-CoV-2 |pdf=|usr=}}{{32371536}} gab.com Text Ultrastructural Evidence for Direct Renal Infection with SARS-CoV-2 JO: J Am Soc Nephrol http://www.kidney.de/pget.php?&tw=1&pmid=32371536 #FOAvip BACKGROUND: A significant fraction of patients with coronavirus disease 2019 (COVID-19) display abnormalities in renal function. Retrospective studies of patients hospitalized with COVID-19 in Wuhan, China, report an incidence of 3%-7% progressing to ARF, a marker of poor prognosis. The cause of the renal failure in COVID-19 is unknown, but one hypothesized mechanism is direct renal infection by the causative virus, SARS-CoV-2. METHODS: We performed an autopsy on a single patient who died of COVID-19 after open repair of an aortic dissection, complicated by hypoxic respiratory failure and oliguric renal failure. We used light and electron microscopy to examine renal tissue for evidence of SARS-CoV-2 within renal cells. RESULTS: Light microscopy of proximal tubules showed geographic isometric vacuolization, corresponding to a focus of tubules with abundant intracellular viral arrays. Individual viruses averaged 76 microm in diameter and had an envelope studded with crown-like, electron-dense spikes. Vacuoles contained double-membrane vesicles suggestive of partially assembled virus. CONCLUSIONS: The presence of viral particles in the renal tubular epithelium that were morphologically identical to SARS-CoV-2, and with viral arrays and other features of virus assembly, provide evidence of a productive direct infection of the kidney by SARS-CoV-2. This finding offers confirmatory evidence that direct renal infection occurs in the setting of AKI in COVID-19. However, the frequency and clinical significance of direct infection in COVID-19 is unclear. Tubular isometric vacuolization observed with light microscopy, which correlates with double-membrane vesicles containing vacuoles observed with electronic microscopy, may be a useful histologic marker for active SARS-CoV-2 infection in kidney biopsy or autopsy specimens. Twit Text FOAVip Ultrastructural Evidence for Direct Renal Infection with SARS-CoV-2 ????J Am Soc Nephrol http://www.kidney.de/pget.php?&tw=1&pmid=32371536 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32371536 #FOAvip English Wikipedia <ref>{{Cite pmid|32371536}}</ref> Ultrastructural Evidence for Direct Renal Infection with SARS-CoV-2 #MMPMID32371536 Farkash EA; Wilson AM; Jentzen JM J Am Soc Nephrol 2020[Aug]; 31 (8): 1683-1687 PMID32371536show ga BACKGROUND: A significant fraction of patients with coronavirus disease 2019 (COVID-19) display abnormalities in renal function. Retrospective studies of patients hospitalized with COVID-19 in Wuhan, China, report an incidence of 3%-7% progressing to ARF, a marker of poor prognosis. The cause of the renal failure in COVID-19 is unknown, but one hypothesized mechanism is direct renal infection by the causative virus, SARS-CoV-2. METHODS: We performed an autopsy on a single patient who died of COVID-19 after open repair of an aortic dissection, complicated by hypoxic respiratory failure and oliguric renal failure. We used light and electron microscopy to examine renal tissue for evidence of SARS-CoV-2 within renal cells. RESULTS: Light microscopy of proximal tubules showed geographic isometric vacuolization, corresponding to a focus of tubules with abundant intracellular viral arrays. Individual viruses averaged 76 microm in diameter and had an envelope studded with crown-like, electron-dense spikes. Vacuoles contained double-membrane vesicles suggestive of partially assembled virus. CONCLUSIONS: The presence of viral particles in the renal tubular epithelium that were morphologically identical to SARS-CoV-2, and with viral arrays and other features of virus assembly, provide evidence of a productive direct infection of the kidney by SARS-CoV-2. This finding offers confirmatory evidence that direct renal infection occurs in the setting of AKI in COVID-19. However, the frequency and clinical significance of direct infection in COVID-19 is unclear. Tubular isometric vacuolization observed with light microscopy, which correlates with double-membrane vesicles containing vacuoles observed with electronic microscopy, may be a useful histologic marker for active SARS-CoV-2 infection in kidney biopsy or autopsy specimens. |Acute Kidney Injury/*complications/mortality[MESH] |Aortic Dissection/surgery[MESH] |Autopsy[MESH] |Betacoronavirus[MESH] |COVID-19[MESH] |Coronavirus Infections/*complications/mortality[MESH] |Epithelial Cells/pathology[MESH] |Humans[MESH] |Kidney Tubules/pathology/ultrastructure/*virology[MESH] |Male[MESH] |Middle Aged[MESH] |Nephritis/physiopathology[MESH] |Pandemics[MESH] |Pneumonia, Viral/*complications/mortality[MESH] |Prognosis[MESH] |Respiratory Insufficiency[MESH] |Retrospective Studies[MESH]Ultrastructural Evidence for Direct Renal Infection with SARS-CoV-2 (epmc).pdf DeepDyve Pubget Overpricing