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Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Int+J+Mol+Sci 2020 ; 21 (9): ä Nephropedia Template TP
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Developments of Riboswitches and Toehold Switches for Molecular Detection-Biosensing and Molecular Diagnostics #MMPMID32366036
Chau THT; Mai DHA; Pham DN; Le HTQ; Lee EY
Int J Mol Sci 2020[Apr]; 21 (9): ä PMID32366036show ga
Riboswitches and toehold switches are considered to have potential for implementation in various fields, i.e., biosensing, metabolic engineering, and molecular diagnostics. The specific binding, programmability, and manipulability of these RNA-based molecules enable their intensive deployments in molecular detection as biosensors for regulating gene expressions, tracking metabolites, or detecting RNA sequences of pathogenic microorganisms. In this review, we will focus on the development of riboswitches and toehold switches in biosensing and molecular diagnostics. This review introduces the operating principles and the notable design features of riboswitches as well as toehold switches. Moreover, we will describe the advances and future directions of riboswitches and toehold switches in biosensing and molecular diagnostics.