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10.1016/j.phrs.2020.104850 http://scihub22266oqcxt.onion/10.1016/j.phrs.2020.104850 32360580!7192119!32360580     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 300.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Pharmacol+Res 2020 ; 158 (ä): 104850 Nephropedia Template TP {{tp|p=32360580|t=2020. Liu Shen capsule shows antiviral and anti-inflammatory abilities against novel coronavirus SARS-CoV-2 via suppression of NF-kappaB signaling pathway |pdf=|usr=}}{{32360580}} gab.com Text Liu Shen capsule shows antiviral and anti-inflammatory abilities against novel coronavirus SARS-CoV-2 via suppression of NF-kappaB signaling pathway JO: Pharmacol Res http://www.kidney.de/pget.php?&tw=1&pmid=32360580 #FOAvip Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has spread worldwide through person-to-person contact, causing a public health emergency of international concern. At present, there is no specific antiviral treatment recommended for SARS-CoV-2 infection. Liu Shen capsule (LS), a traditional Chinese medicine, has been proven to have a wide spectrum of pharmacological properties, such as anti-inflammatory, antiviral and immunomodulatory activities. However, little is known about the antiviral effect of LS against SARS-CoV-2. Herein, the study was designed to investigate the antiviral activity of SARS-CoV-2 and its potential effect in regulating the host's immune response. The inhibitory effect of LS against SARS-CoV-2 replication in Vero E6 cells was evaluated by using the cytopathic effect (CPE) and plaque reduction assay. The number of virions of SARS-CoV-2 was observed under transmission electron microscope after treatment with LS. Proinflammatory cytokine expression levels upon SARS-CoV-2 infection in Huh-7 cells were measured by real-time quantitative PCR assays. The results showed that LS could significantly inhibit SARS-CoV-2 replication in Vero E6 cells, and reduce the number of virus particles and it could markedly reduce pro-inflammatory cytokines (TNF-alpha, IL-6, IL-1beta, IL-8, CCL-2/MCP-1 and CXCL-10/IP-10) production at the mRNA levels. Moreover, the expression of the key proteins in the NF-kappaB/MAPK signaling pathway was detected by western blot and it was found that LS could inhibit the expression of p-NF-kappaB p65, p-IkappaBalpha and p-p38 MAPK, while increasing the expression of IkappaBalpha. These findings indicate that LS could inhibit SARS-CoV-2 virus infection via downregulating the expression of inflammatory cytokines induced virus and regulating the activity of NF-kappaB/MAPK signaling pathway in vitro, making its promising candidate treatment for controlling COVID-19 disease. Twit Text FOAVip Liu Shen capsule shows antiviral and anti-inflammatory abilities against novel coronavirus SARS-CoV-2 via suppression of NF-kappaB signaling pathway ????Pharmacol Res http://www.kidney.de/pget.php?&tw=1&pmid=32360580 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32360580 #FOAvip English Wikipedia <ref>{{Cite pmid|32360580}}</ref> Liu Shen capsule shows antiviral and anti-inflammatory abilities against novel coronavirus SARS-CoV-2 via suppression of NF-kappaB signaling pathway #MMPMID32360580 Ma Q; Pan W; Li R; Liu B; Li C; Xie Y; Wang Z; Zhao J; Jiang H; Huang J; Shi Y; Dai J; Zheng K; Li X; Yang Z Pharmacol Res 2020[Aug]; 158 (ä): 104850 PMID32360580show ga Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has spread worldwide through person-to-person contact, causing a public health emergency of international concern. At present, there is no specific antiviral treatment recommended for SARS-CoV-2 infection. Liu Shen capsule (LS), a traditional Chinese medicine, has been proven to have a wide spectrum of pharmacological properties, such as anti-inflammatory, antiviral and immunomodulatory activities. However, little is known about the antiviral effect of LS against SARS-CoV-2. Herein, the study was designed to investigate the antiviral activity of SARS-CoV-2 and its potential effect in regulating the host's immune response. The inhibitory effect of LS against SARS-CoV-2 replication in Vero E6 cells was evaluated by using the cytopathic effect (CPE) and plaque reduction assay. The number of virions of SARS-CoV-2 was observed under transmission electron microscope after treatment with LS. Proinflammatory cytokine expression levels upon SARS-CoV-2 infection in Huh-7 cells were measured by real-time quantitative PCR assays. The results showed that LS could significantly inhibit SARS-CoV-2 replication in Vero E6 cells, and reduce the number of virus particles and it could markedly reduce pro-inflammatory cytokines (TNF-alpha, IL-6, IL-1beta, IL-8, CCL-2/MCP-1 and CXCL-10/IP-10) production at the mRNA levels. Moreover, the expression of the key proteins in the NF-kappaB/MAPK signaling pathway was detected by western blot and it was found that LS could inhibit the expression of p-NF-kappaB p65, p-IkappaBalpha and p-p38 MAPK, while increasing the expression of IkappaBalpha. These findings indicate that LS could inhibit SARS-CoV-2 virus infection via downregulating the expression of inflammatory cytokines induced virus and regulating the activity of NF-kappaB/MAPK signaling pathway in vitro, making its promising candidate treatment for controlling COVID-19 disease. |Adenosine Monophosphate/analogs & derivatives/pharmacology[MESH] |Alanine/analogs & derivatives/pharmacology[MESH] |Animals[MESH] |Anti-Inflammatory Agents/pharmacology[MESH] |Antiviral Agents/pharmacology[MESH] |Betacoronavirus/*drug effects[MESH] |COVID-19[MESH] |Cell Proliferation/drug effects[MESH] |Cells, Cultured[MESH] |Chlorocebus aethiops[MESH] |Complex Mixtures/*pharmacology[MESH] |Coronavirus Infections/virology[MESH] |Humans[MESH] |Inflammation Mediators/metabolism[MESH] |NF-kappa B/*antagonists & inhibitors/*metabolism[MESH] |Pandemics[MESH] |Pneumonia, Viral/virology[MESH] |SARS-CoV-2[MESH] |Signal Transduction/*drug effects[MESH]Liu Shen capsule shows antiviral and anti-inflammatory abilities again(epmc).pdf DeepDyve Pubget Overpricing