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10.13345/j.cjb.200088 http://scihub22266oqcxt.onion/10.13345/j.cjb.200088 32347067!ä!32347067 Warning: Undefined variable $yww in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 538 Warning: Undefined variable $yww in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 538       Sheng+Wu+Gong+Cheng+Xue+Bao 2020 ; 36 (4): 732-739 Nephropedia Template TP {{tp|p=32347067|t=2020. Optimization of a fluorescent qPCR detection for RNA of SARS-CoV-2 |pdf=|usr=}}{{32347067}} gab.com Text Optimization of a fluorescent qPCR detection for RNA of SARS-CoV-2 JO: Sheng Wu Gong Cheng Xue Bao http://www.kidney.de/pget.php?&tw=1&pmid=32347067 #FOAvip We optimized a fluorescent quantitative polymerase chain reaction (qPCR) assay system for rapid and real time detection of SARS-CoV-2 RNA. The results show that the lowest dilution of RNA samples used for the detection of SARS-CoV-2 RNA could reach 1/10 000 (the initial value is set as 10 ng/muL). Moreover, the cycle threshold (Ct) for samples of clinically diagnosed COVID-19 was lower than 35 or 40. The sensitivity of this method was satisfactory. The results were consistent with those of the COVID-19 detection kit on the market under the same conditions, but the number of cycles required was shortened by about 2. Therefore, the optimized assay developed in this study can be used in screening and early clinical diagnosis. Our work provides a tool to facilitate rapid clinical diagnosis of COVID-19. Twit Text FOAVip Optimization of a fluorescent qPCR detection for RNA of SARS-CoV-2 ????Sheng Wu Gong Cheng Xue Bao http://www.kidney.de/pget.php?&tw=1&pmid=32347067 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32347067 #FOAvip English Wikipedia <ref>{{Cite pmid|32347067}}</ref> Optimization of a fluorescent qPCR detection for RNA of SARS-CoV-2 #MMPMID32347067 Li X; Liu J; Liu Q; Yu L; Wu S; Yin X Sheng Wu Gong Cheng Xue Bao 2020[Apr]; 36 (4): 732-739 PMID32347067show ga We optimized a fluorescent quantitative polymerase chain reaction (qPCR) assay system for rapid and real time detection of SARS-CoV-2 RNA. The results show that the lowest dilution of RNA samples used for the detection of SARS-CoV-2 RNA could reach 1/10 000 (the initial value is set as 10 ng/muL). Moreover, the cycle threshold (Ct) for samples of clinically diagnosed COVID-19 was lower than 35 or 40. The sensitivity of this method was satisfactory. The results were consistent with those of the COVID-19 detection kit on the market under the same conditions, but the number of cycles required was shortened by about 2. Therefore, the optimized assay developed in this study can be used in screening and early clinical diagnosis. Our work provides a tool to facilitate rapid clinical diagnosis of COVID-19. |Betacoronavirus/*genetics/*isolation & purification[MESH] |COVID-19[MESH] |Coronavirus Infections/*diagnosis/*virology[MESH] |Early Diagnosis[MESH] |Humans[MESH] |Pandemics[MESH] |Pneumonia, Viral/*diagnosis/*virology[MESH] |Polymerase Chain Reaction/*methods/*standards[MESH] |RNA, Viral/*analysis/genetics[MESH] |SARS-CoV-2[MESH] |Sensitivity and Specificity[MESH] Optimization of a fluorescent qPCR detection for RNA of SARS-CoV-2 (epmc).pdf DeepDyve Pubget Overpricing