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10.18632/aging.103171 http://scihub22266oqcxt.onion/10.18632/aging.103171 32345770!7202536!32345770     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 261.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Aging+(Albany+NY) 2020 ; 12 (8): 7603-7613 Nephropedia Template TP {{tp|p=32345770|t=2020. LUCS: a high-resolution nucleic acid sequencing tool for accurate long-read analysis of individual DNA molecules |pdf=|usr=}}{{32345770}} gab.com Text LUCS: a high-resolution nucleic acid sequencing tool for accurate long-read analysis of individual DNA molecules JO: Aging (Albany NY) http://www.kidney.de/pget.php?&tw=1&pmid=32345770 #FOAvip Nucleic acid sequence analyses are fundamental to all aspects of biological research, spanning aging, mitochondrial DNA (mtDNA) and cancer, as well as microbial and viral evolution. Over the past several years, significant improvements in DNA sequencing, including consensus sequence analysis, have proven invaluable for high-throughput studies. However, all current DNA sequencing platforms have limited utility for studies of complex mixtures or of individual long molecules, the latter of which is crucial to understanding evolution and consequences of single nucleotide variants and their combinations. Here we report a new technology termed LUCS (Long-molecule UMI-driven Consensus Sequencing), in which reads from third-generation sequencing are aggregated by unique molecular identifiers (UMIs) specific for each individual DNA molecule. This enables in-silico reconstruction of highly accurate consensus reads of each DNA molecule independent of other molecules in the sample. Additionally, use of two UMIs enables detection of artificial recombinants (chimeras). As proof of concept, we show that application of LUCS to assessment of mitochondrial genomes in complex mixtures from single cells was associated with an error rate of 1X10(-4) errors/nucleotide. Thus, LUCS represents a major step forward in DNA sequencing that offers high-throughput capacity and high-accuracy reads in studies of long DNA templates and nucleotide variants in heterogenous samples. Twit Text FOAVip LUCS: a high-resolution nucleic acid sequencing tool for accurate long-read analysis of individual DNA molecules ????Aging (Albany NY) http://www.kidney.de/pget.php?&tw=1&pmid=32345770 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32345770 #FOAvip English Wikipedia <ref>{{Cite pmid|32345770}}</ref> LUCS: a high-resolution nucleic acid sequencing tool for accurate long-read analysis of individual DNA molecules #MMPMID32345770 Annis S; Fleischmann Z; Logan R; Mullin-Bernstein Z; Franco M; Saurich J; Tilly JL; Woods DC; Khrapko K Aging (Albany NY) 2020[Apr]; 12 (8): 7603-7613 PMID32345770show ga Nucleic acid sequence analyses are fundamental to all aspects of biological research, spanning aging, mitochondrial DNA (mtDNA) and cancer, as well as microbial and viral evolution. Over the past several years, significant improvements in DNA sequencing, including consensus sequence analysis, have proven invaluable for high-throughput studies. However, all current DNA sequencing platforms have limited utility for studies of complex mixtures or of individual long molecules, the latter of which is crucial to understanding evolution and consequences of single nucleotide variants and their combinations. Here we report a new technology termed LUCS (Long-molecule UMI-driven Consensus Sequencing), in which reads from third-generation sequencing are aggregated by unique molecular identifiers (UMIs) specific for each individual DNA molecule. This enables in-silico reconstruction of highly accurate consensus reads of each DNA molecule independent of other molecules in the sample. Additionally, use of two UMIs enables detection of artificial recombinants (chimeras). As proof of concept, we show that application of LUCS to assessment of mitochondrial genomes in complex mixtures from single cells was associated with an error rate of 1X10(-4) errors/nucleotide. Thus, LUCS represents a major step forward in DNA sequencing that offers high-throughput capacity and high-accuracy reads in studies of long DNA templates and nucleotide variants in heterogenous samples. |*Mutation[MESH] |DNA/analysis/*genetics[MESH] |High-Throughput Nucleotide Sequencing/*methods[MESH] |Humans[MESH]LUCS: a high-resolution nucleic acid sequencing tool for accurate long(epmc).pdf DeepDyve Pubget Overpricing