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10.3390/ijms21083004

http://scihub22266oqcxt.onion/10.3390/ijms21083004
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32344568!7215906!32344568
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suck abstract from ncbi

pmid32344568      Int+J+Mol+Sci 2020 ; 21 (8): ä
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  • RT-qPCR Testing of SARS-CoV-2: A Primer #MMPMID32344568
  • Bustin SA; Nolan T
  • Int J Mol Sci 2020[Apr]; 21 (8): ä PMID32344568show ga
  • Testing for the presence of coronavirus is an essential diagnostic tool for monitoring and managing the current COVID-19 pandemic. The only reliable test in current use for testing acute infection targets the genome of SARS-CoV-2, and the most widely used method is quantitative fluorescence-based reverse transcription polymerase chain reaction (RT-qPCR). Despite its ubiquity, there is a significant amount of uncertainty about how this test works, potential throughput and reliability. This has resulted in widespread misrepresentation of the problems faced using this test during the current COVID-19 epidemic. This primer provides simple, straightforward and impartial information about RT-qPCR.
  • |*DNA Primers[MESH]
  • |Betacoronavirus/genetics/*isolation & purification[MESH]
  • |COVID-19[MESH]
  • |COVID-19 Testing[MESH]
  • |Clinical Laboratory Techniques/*methods[MESH]
  • |Coronavirus Infections/diagnosis/virology[MESH]
  • |Pandemics[MESH]
  • |Pneumonia, Viral/diagnosis/virology[MESH]
  • |RNA, Viral/genetics/isolation & purification[MESH]
  • |Real-Time Polymerase Chain Reaction/*methods[MESH]


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