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10.1371/journal.pone.0231723 http://scihub22266oqcxt.onion/10.1371/journal.pone.0231723 32343704!7188253!32343704     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       PLoS+One 2020 ; 15 (4): e0231723 Nephropedia Template TP {{tp|p=32343704|t=2020. Immune responses to porcine epidemic diarrhea virus (PEDV) in swine and protection against subsequent infection |pdf=|usr=}}{{32343704}} gab.com Text Immune responses to porcine epidemic diarrhea virus (PEDV) in swine and protection against subsequent infection JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=32343704 #FOAvip Understanding the immune responses against Porcine epidemic diarrhea virus (PEDV) is important to prevent infection and to design control strategies. We evaluated both systemic and mucosal immune responses to PEDV in pigs and assessed if prior exposure to virus protects against re-infection. Three-week-old pigs were infected with PEDV and immune response in blood, intestine, and mesenteric lymph node (MLN) was evaluated. At 30 dpi, virus exposed pigs were challenged with a field isolate of PEDV and immune response at 5 d post challenge was evaluated. We found that PEDV RNA persists in the intestine even after fecal shedding of the virus was stopped at 28 dpi and pigs previously exposed to PEDV are protected from virus shedding after re-infection. PEDV infection induced both humoral and cell mediated immune response with an increase in PEDV specific IgA and IgG antibodies in intestine and serum. Flow cytometry analysis showed a significantly higher frequency of B cells and lower frequency of T cells at 4 dpi. The frequency of CD4/CD8 double positive (DP) memory T cells was significantly increased in the MLN of challenged animals. These studies may provide further insights into understanding the mucosal immune response to PEDV and its role in protection against disease. Twit Text FOAVip Immune responses to porcine epidemic diarrhea virus (PEDV) in swine and protection against subsequent infection ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=32343704 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32343704 #FOAvip English Wikipedia <ref>{{Cite pmid|32343704}}</ref> Immune responses to porcine epidemic diarrhea virus (PEDV) in swine and protection against subsequent infection #MMPMID32343704 Krishna VD; Kim Y; Yang M; Vannucci F; Molitor T; Torremorell M; Cheeran MC PLoS One 2020[]; 15 (4): e0231723 PMID32343704show ga Understanding the immune responses against Porcine epidemic diarrhea virus (PEDV) is important to prevent infection and to design control strategies. We evaluated both systemic and mucosal immune responses to PEDV in pigs and assessed if prior exposure to virus protects against re-infection. Three-week-old pigs were infected with PEDV and immune response in blood, intestine, and mesenteric lymph node (MLN) was evaluated. At 30 dpi, virus exposed pigs were challenged with a field isolate of PEDV and immune response at 5 d post challenge was evaluated. We found that PEDV RNA persists in the intestine even after fecal shedding of the virus was stopped at 28 dpi and pigs previously exposed to PEDV are protected from virus shedding after re-infection. PEDV infection induced both humoral and cell mediated immune response with an increase in PEDV specific IgA and IgG antibodies in intestine and serum. Flow cytometry analysis showed a significantly higher frequency of B cells and lower frequency of T cells at 4 dpi. The frequency of CD4/CD8 double positive (DP) memory T cells was significantly increased in the MLN of challenged animals. These studies may provide further insights into understanding the mucosal immune response to PEDV and its role in protection against disease. |Animals[MESH] |Antibodies, Viral/*analysis/immunology/metabolism[MESH] |B-Lymphocytes/immunology[MESH] |Coronavirus Infections/blood/*immunology/veterinary/virology[MESH] |Diarrhea/blood/*immunology/veterinary/virology[MESH] |Disease Resistance/immunology[MESH] |Feces/microbiology[MESH] |Immunity, Cellular[MESH] |Immunity, Humoral[MESH] |Immunity, Mucosal[MESH] |Immunoglobulin A/analysis/immunology/metabolism[MESH] |Immunoglobulin G/analysis/immunology/metabolism[MESH] |Intestinal Mucosa/immunology/metabolism[MESH] |Porcine epidemic diarrhea virus/genetics/*immunology/isolation & purification[MESH] |RNA, Viral/isolation & purification[MESH] |Swine[MESH] |T-Lymphocytes/immunology[MESH]Immune responses to porcine epidemic diarrhea virus (PEDV) in swine an(epmc).pdf DeepDyve Pubget Overpricing