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10.1039/d0lc00304b http://scihub22266oqcxt.onion/10.1039/d0lc00304b 32334422!ä!32334422 Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=32334422&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Lab+Chip 2020 ; 20 (9): 1621-1627 Nephropedia Template TP {{tp|p=32334422|t=2020. Smartphone-based multiplex 30-minute nucleic acid test of live virus from nasal swab extract |pdf=|usr=}}{{32334422}} gab.com Text Smartphone-based multiplex 30-minute nucleic acid test of live virus from nasal swab extract JO: Lab Chip http://www.kidney.de/pget.php?&tw=1&pmid=32334422 #FOAvip Rapid, sensitive and specific detection and reporting of infectious pathogens is important for patient management and epidemic surveillance. We demonstrated a point-of-care system integrated with a smartphone for detecting live virus from nasal swab media, using a panel of equine respiratory infectious diseases as a model system for corresponding human diseases such as COVID-19. Specific nucleic acid sequences of five pathogens were amplified by loop-mediated isothermal amplification on a microfluidic chip and detected at the end of reactions by the smartphone. Pathogen-spiked horse nasal swab samples were correctly diagnosed using our system, with a limit of detection comparable to that of the traditional lab-based test, polymerase chain reaction, with results achieved in approximately 30 minutes. Twit Text FOAVip Smartphone-based multiplex 30-minute nucleic acid test of live virus from nasal swab extract ????Lab Chip http://www.kidney.de/pget.php?&tw=1&pmid=32334422 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32334422 #FOAvip English Wikipedia <ref>{{Cite pmid|32334422}}</ref> Smartphone-based multiplex 30-minute nucleic acid test of live virus from nasal swab extract #MMPMID32334422 Sun F; Ganguli A; Nguyen J; Brisbin R; Shanmugam K; Hirschberg DL; Wheeler MB; Bashir R; Nash DM; Cunningham BT Lab Chip 2020[May]; 20 (9): 1621-1627 PMID32334422show ga Rapid, sensitive and specific detection and reporting of infectious pathogens is important for patient management and epidemic surveillance. We demonstrated a point-of-care system integrated with a smartphone for detecting live virus from nasal swab media, using a panel of equine respiratory infectious diseases as a model system for corresponding human diseases such as COVID-19. Specific nucleic acid sequences of five pathogens were amplified by loop-mediated isothermal amplification on a microfluidic chip and detected at the end of reactions by the smartphone. Pathogen-spiked horse nasal swab samples were correctly diagnosed using our system, with a limit of detection comparable to that of the traditional lab-based test, polymerase chain reaction, with results achieved in approximately 30 minutes. |*Lab-On-A-Chip Devices[MESH] |*Smartphone[MESH] |Animals[MESH] |Betacoronavirus/isolation & purification[MESH] |COVID-19 Testing[MESH] |Clinical Laboratory Techniques/methods[MESH] |Coronavirus Infections/diagnosis[MESH] |Herpesvirus 1, Equid/isolation & purification[MESH] |Herpesvirus 4, Equid/isolation & purification[MESH] |Horse Diseases/*diagnosis/microbiology/virology[MESH] |Horses[MESH] |Influenza A Virus, H3N8 Subtype/isolation & purification[MESH] |Mobile Applications[MESH] |Molecular Diagnostic Techniques/*methods[MESH] |Nose/microbiology/virology[MESH] |Nucleic Acid Amplification Techniques/*methods[MESH] |Point-of-Care Systems[MESH] |Respiration Disorders/diagnosis/microbiology/*veterinary/virology[MESH] |SARS-CoV-2[MESH]Smartphone-based multiplex 30-minute nucleic acid test of live virus f(epmc).pdf DeepDyve Pubget Overpricing