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10.1016/j.cell.2020.04.011 http://scihub22266oqcxt.onion/10.1016/j.cell.2020.04.011 32330414!7179501!32330414     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=32330414&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       Cell 2020 ; 181 (4): 914-921.e10 Nephropedia Template TP {{tp|p=32330414|t=2020. The Architecture of SARS-CoV-2 Transcriptome |pdf=|usr=}}{{32330414}} gab.com Text The Architecture of SARS-CoV-2 Transcriptome JO: Cell http://www.kidney.de/pget.php?&tw=1&pmid=32330414 #FOAvip SARS-CoV-2 is a betacoronavirus responsible for the COVID-19 pandemic. Although the SARS-CoV-2 genome was reported recently, its transcriptomic architecture is unknown. Utilizing two complementary sequencing techniques, we present a high-resolution map of the SARS-CoV-2 transcriptome and epitranscriptome. DNA nanoball sequencing shows that the transcriptome is highly complex owing to numerous discontinuous transcription events. In addition to the canonical genomic and 9 subgenomic RNAs, SARS-CoV-2 produces transcripts encoding unknown ORFs with fusion, deletion, and/or frameshift. Using nanopore direct RNA sequencing, we further find at least 41 RNA modification sites on viral transcripts, with the most frequent motif, AAGAA. Modified RNAs have shorter poly(A) tails than unmodified RNAs, suggesting a link between the modification and the 3' tail. Functional investigation of the unknown transcripts and RNA modifications discovered in this study will open new directions to our understanding of the life cycle and pathogenicity of SARS-CoV-2. Twit Text FOAVip The Architecture of SARS-CoV-2 Transcriptome ????Cell http://www.kidney.de/pget.php?&tw=1&pmid=32330414 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32330414 #FOAvip English Wikipedia <ref>{{Cite pmid|32330414}}</ref> The Architecture of SARS-CoV-2 Transcriptome #MMPMID32330414 Kim D; Lee JY; Yang JS; Kim JW; Kim VN; Chang H Cell 2020[May]; 181 (4): 914-921.e10 PMID32330414show ga SARS-CoV-2 is a betacoronavirus responsible for the COVID-19 pandemic. Although the SARS-CoV-2 genome was reported recently, its transcriptomic architecture is unknown. Utilizing two complementary sequencing techniques, we present a high-resolution map of the SARS-CoV-2 transcriptome and epitranscriptome. DNA nanoball sequencing shows that the transcriptome is highly complex owing to numerous discontinuous transcription events. In addition to the canonical genomic and 9 subgenomic RNAs, SARS-CoV-2 produces transcripts encoding unknown ORFs with fusion, deletion, and/or frameshift. Using nanopore direct RNA sequencing, we further find at least 41 RNA modification sites on viral transcripts, with the most frequent motif, AAGAA. Modified RNAs have shorter poly(A) tails than unmodified RNAs, suggesting a link between the modification and the 3' tail. Functional investigation of the unknown transcripts and RNA modifications discovered in this study will open new directions to our understanding of the life cycle and pathogenicity of SARS-CoV-2. |*Transcriptome[MESH] |Animals[MESH] |Betacoronavirus/*genetics[MESH] |Chlorocebus aethiops[MESH] |Epigenesis, Genetic[MESH] |RNA Processing, Post-Transcriptional[MESH] |RNA, Viral/*genetics[MESH] |SARS-CoV-2[MESH] |Sequence Analysis, RNA[MESH]The Architecture of SARS-CoV-2 Transcriptome (epmc).pdf DeepDyve Pubget Overpricing