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10.1016/j.snb.2020.128160

http://scihub22266oqcxt.onion/10.1016/j.snb.2020.128160
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suck abstract from ncbi


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pmid32322135      Sens+Actuators+B+Chem 2020 ; 316 (ä): 128160
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  • Rapid detection of anti-H5 avian influenza virus antibody by fluorescence polarization immunoassay using a portable fluorescence polarization analyzer #MMPMID32322135
  • Nishiyama K; Takeda Y; Maeki M; Ishida A; Tani H; Shigemura K; Hibara A; Yonezawa Y; Imai K; Ogawa H; Tokeshi M
  • Sens Actuators B Chem 2020[Aug]; 316 (ä): 128160 PMID32322135show ga
  • A rapid, facile and selective detection of anti-H5 subtype avian influenza virus (AIV) antibody in serum by fluorescence polarization immunoassay (FPIA) was achieved. A fragment of recombinant H5 subtype AIV hemagglutinin was produced and labeled with fluorescein to use it as a labeled antigen in FPIA. This labeled antigen was mixed with anti-AIV sera (H1-H16 subtypes) and FP of the mixture was measured using a portable FP analyzer on a microdevice. It was found that FP increased in proportion to the concentration of anti-H5 AIV antibody (serum) and was significantly higher than FP obtained with the other sera. The selective detection of anti-H5 subtype AIV antibody was confirmed. The required volume of original sample was 2?muL and analysis time was within 20?min. This detection system realizes an efficient on-site diagnosis and surveillance of AIV.
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