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10.1080/22221751.2020.1756700 http://scihub22266oqcxt.onion/10.1080/22221751.2020.1756700 32301390!7241555!32301390     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Emerg+Microbes+Infect 2020 ; 9 (1): 837-842 Nephropedia Template TP {{tp|p=32301390|t=2020. Attenuated SARS-CoV-2 variants with deletions at the S1/S2 junction |pdf=|usr=}}{{32301390}} gab.com Text Attenuated SARS-CoV-2 variants with deletions at the S1/S2 junction JO: Emerg Microbes Infect http://www.kidney.de/pget.php?&tw=1&pmid=32301390 #FOAvip The emergence of SARS-CoV-2 has led to the current global coronavirus pandemic and more than one million infections since December 2019. The exact origin of SARS-CoV-2 remains elusive, but the presence of a distinct motif in the S1/S2 junction region suggests the possible acquisition of cleavage site(s) in the spike protein that promoted cross-species transmission. Through plaque purification of Vero-E6 cultured SARS-CoV-2, we found a series of variants which contain 15-30-bp deletions (Del-mut) or point mutations respectively at the S1/S2 junction. Examination of the original clinical specimen from which the isolate was derived, and 26 additional SARS-CoV-2 positive clinical specimens, failed to detect these variants. Infection of hamsters shows that one of the variants (Del-mut-1) which carries deletion of 10 amino acids (30bp) does not cause the body weight loss or more severe pathological changes in the lungs that is associated with wild type virus infection. We suggest that the unique cleavage motif promoting SARS-CoV-2 infection in humans may be under strong selective pressure, given that replication in permissive Vero-E6 cells leads to the loss of this adaptive function. It would be important to screen the prevalence of these variants in asymptomatic infected cases. The potential of the Del-mut variants as an attenuated vaccine or laboratory tool should be evaluated. Twit Text FOAVip Attenuated SARS-CoV-2 variants with deletions at the S1/S2 junction ????Emerg Microbes Infect http://www.kidney.de/pget.php?&tw=1&pmid=32301390 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32301390 #FOAvip English Wikipedia <ref>{{Cite pmid|32301390}}</ref> Attenuated SARS-CoV-2 variants with deletions at the S1/S2 junction #MMPMID32301390 Lau SY; Wang P; Mok BW; Zhang AJ; Chu H; Lee AC; Deng S; Chen P; Chan KH; Song W; Chen Z; To KK; Chan JF; Yuen KY; Chen H Emerg Microbes Infect 2020[Dec]; 9 (1): 837-842 PMID32301390show ga The emergence of SARS-CoV-2 has led to the current global coronavirus pandemic and more than one million infections since December 2019. The exact origin of SARS-CoV-2 remains elusive, but the presence of a distinct motif in the S1/S2 junction region suggests the possible acquisition of cleavage site(s) in the spike protein that promoted cross-species transmission. Through plaque purification of Vero-E6 cultured SARS-CoV-2, we found a series of variants which contain 15-30-bp deletions (Del-mut) or point mutations respectively at the S1/S2 junction. Examination of the original clinical specimen from which the isolate was derived, and 26 additional SARS-CoV-2 positive clinical specimens, failed to detect these variants. Infection of hamsters shows that one of the variants (Del-mut-1) which carries deletion of 10 amino acids (30bp) does not cause the body weight loss or more severe pathological changes in the lungs that is associated with wild type virus infection. We suggest that the unique cleavage motif promoting SARS-CoV-2 infection in humans may be under strong selective pressure, given that replication in permissive Vero-E6 cells leads to the loss of this adaptive function. It would be important to screen the prevalence of these variants in asymptomatic infected cases. The potential of the Del-mut variants as an attenuated vaccine or laboratory tool should be evaluated. |*Disease Models, Animal[MESH] |*Mesocricetus[MESH] |*Sequence Deletion[MESH] |Amino Acid Sequence[MESH] |Animals[MESH] |Base Sequence[MESH] |COVID-19[MESH] |Cell Line[MESH] |Chlorocebus aethiops[MESH] |Coronavirus Infections/*pathology/virology[MESH] |Female[MESH] |Host Specificity[MESH] |Humans[MESH] |Lung/pathology[MESH] |Male[MESH] |Pandemics[MESH] |Pneumonia, Viral/*pathology/virology[MESH] |Severe acute respiratory syndrome-related coronavirus/*genetics/growth & development/isolation & purification/*pathogenicity[MESH] |Spike Glycoprotein, Coronavirus/chemistry/*genetics[MESH] |Vero Cells[MESH]Attenuated SARS-CoV-2 variants with deletions at the S1/S2 junction (epmc).pdf DeepDyve Pubget Overpricing