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CRISPR-Cas12-based detection of SARS-CoV-2 #MMPMID32300245
Broughton JP; Deng X; Yu G; Fasching CL; Servellita V; Singh J; Miao X; Streithorst JA; Granados A; Sotomayor-Gonzalez A; Zorn K; Gopez A; Hsu E; Gu W; Miller S; Pan CY; Guevara H; Wadford DA; Chen JS; Chiu CY
Nat Biotechnol 2020[Jul]; 38 (7): 870-874 PMID32300245show ga
An outbreak of betacoronavirus severe acute respiratory syndrome (SARS)-CoV-2 began in Wuhan, China in December 2019. COVID-19, the disease associated with SARS-CoV-2 infection, rapidly spread to produce a global pandemic. We report development of a rapid (<40 min), easy-to-implement and accurate CRISPR-Cas12-based lateral flow assay for detection of SARS-CoV-2 from respiratory swab RNA extracts. We validated our method using contrived reference samples and clinical samples from patients in the United States, including 36 patients with COVID-19 infection and 42 patients with other viral respiratory infections. Our CRISPR-based DETECTR assay provides a visual and faster alternative to the US Centers for Disease Control and Prevention SARS-CoV-2 real-time RT-PCR assay, with 95% positive predictive agreement and 100% negative predictive agreement.
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Nat+Biotechnol 2020 ; 38 (7): 870-874
