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10.3201/eid2607.201285

http://scihub22266oqcxt.onion/10.3201/eid2607.201285
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32294051!7323516!32294051
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suck abstract from ncbi


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pmid32294051      Emerg+Infect+Dis 2020 ; 26 (7): 1633-1635
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  • Triplex Real-Time RT-PCR for Severe Acute Respiratory Syndrome Coronavirus 2 #MMPMID32294051
  • Waggoner JJ; Stittleburg V; Pond R; Saklawi Y; Sahoo MK; Babiker A; Hussaini L; Kraft CS; Pinsky BA; Anderson EJ; Rouphael N
  • Emerg Infect Dis 2020[Jul]; 26 (7): 1633-1635 PMID32294051show ga
  • Most reverse transcription PCR protocols for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) include 2-3 targets for detection. We developed a triplex, real-time reverse transcription PCR for SARS-CoV-2 that maintained clinical performance compared with singleplex assays. This protocol could streamline detection and decrease reagent use during current high SARS-CoV-2 testing demands.
  • |Betacoronavirus/genetics/*isolation & purification[MESH]
  • |Humans[MESH]
  • |Multiplex Polymerase Chain Reaction/*methods[MESH]
  • |Nasopharynx/virology[MESH]
  • |Real-Time Polymerase Chain Reaction/*methods[MESH]


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