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10.2807/1560-7917.ES.2020.25.14.2000398 http://scihub22266oqcxt.onion/10.2807/1560-7917.ES.2020.25.14.2000398 32290902!7160440!32290902     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=32290902&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       Euro+Surveill 2020 ; 25 (14): ? Nephropedia Template TP {{tp|p=32290902|t=2020. An alternative workflow for molecular detection of SARS-CoV-2 - escape from the NA extraction kit-shortage, Copenhagen, Denmark, March 2020 |pdf=|usr=}}{{32290902}} gab.com Text An alternative workflow for molecular detection of SARS-CoV-2 - escape from the NA extraction kit-shortage, Copenhagen, Denmark, March 2020 JO: Euro Surveill http://www.kidney.de/pget.php?&tw=1&pmid=32290902 #FOAvip The World Health Organization has declared COVID-19 caused by the newly discovered SARS-CoV-2 a pandemic. Due to growing demand for reagents and/or kits to extract SARS-CoV-2 RNA for subsequent RT-qPCR diagnostics, there is a worldwide risk of shortages. With a detection sensitivity of 97.4% (95% CI: 86.2-99.9%), we describe a simple, fast, alternative workflow for molecular detection of SARS-CoV-2, where samples are simply heat-processed for 5 min at 98 degrees C before a commonly-used RT-qPCR procedure. Twit Text FOAVip An alternative workflow for molecular detection of SARS-CoV-2 - escape from the NA extraction kit-shortage, Copenhagen, Denmark, March 2020 ????Euro Surveill http://www.kidney.de/pget.php?&tw=1&pmid=32290902 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32290902 #FOAvip English Wikipedia <ref>{{Cite pmid|32290902}}</ref> An alternative workflow for molecular detection of SARS-CoV-2 - escape from the NA extraction kit-shortage, Copenhagen, Denmark, March 2020 #MMPMID32290902 Fomsgaard AS; Rosenstierne MW Euro Surveill 2020[Apr]; 25 (14): ? PMID32290902show ga The World Health Organization has declared COVID-19 caused by the newly discovered SARS-CoV-2 a pandemic. Due to growing demand for reagents and/or kits to extract SARS-CoV-2 RNA for subsequent RT-qPCR diagnostics, there is a worldwide risk of shortages. With a detection sensitivity of 97.4% (95% CI: 86.2-99.9%), we describe a simple, fast, alternative workflow for molecular detection of SARS-CoV-2, where samples are simply heat-processed for 5 min at 98 degrees C before a commonly-used RT-qPCR procedure. |Betacoronavirus[MESH] |COVID-19[MESH] |Coronavirus Infections/*diagnosis[MESH] |Coronavirus/*genetics/*isolation & purification[MESH] |Denmark[MESH] |Humans[MESH] |Oropharynx/*virology[MESH] |Pandemics[MESH] |Pneumonia, Viral/*diagnosis[MESH] |RNA, Viral/*analysis/genetics/isolation & purification[MESH] |Reverse Transcriptase Polymerase Chain Reaction/*methods[MESH] |SARS-CoV-2[MESH] |Sensitivity and Specificity[MESH] |Severe Acute Respiratory Syndrome/diagnosis[MESH]An alternative workflow for molecular detection of SARS-CoV-2 - escape(epmc).pdf DeepDyve Pubget Overpricing