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10.1128/AAC.00086-20 http://scihub22266oqcxt.onion/10.1128/AAC.00086-20 32284379!7269492!32284379     free     free     free Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 209.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Antimicrob+Agents+Chemother 2020 ; 64 (6): ä Nephropedia Template TP {{tp|p=32284379|t=2020. Novel Ionophores Active against La Crosse Virus Identified through Rapid Antiviral Screening |pdf=|usr=}}{{32284379}} gab.com Text Novel Ionophores Active against La Crosse Virus Identified through Rapid Antiviral Screening JO: Antimicrob Agents Chemother http://www.kidney.de/pget.php?&tw=1&pmid=32284379 #FOAvip Bunyaviruses are significant human pathogens, causing diseases ranging from hemorrhagic fevers to encephalitis. Among these viruses, La Crosse virus (LACV), a member of the California serogroup, circulates in the eastern and midwestern United States. While LACV infection is often asymptomatic, dozens of cases of encephalitis are reported yearly. Unfortunately, no antivirals have been approved to treat LACV infection. Here, we developed a method to rapidly test potential antivirals against LACV infection. From this screen, we identified several potential antiviral molecules, including known antivirals. Additionally, we identified many novel antivirals that exhibited antiviral activity without affecting cellular viability. Valinomycin, a potassium ionophore, was among our top targets. We found that valinomycin exhibited potent anti-LACV activity in multiple cell types in a dose-dependent manner. Valinomycin did not affect particle stability or infectivity, suggesting that it may preclude virus replication by altering cellular potassium ions, a known determinant of LACV entry. We extended these results to other ionophores and found that the antiviral activity of valinomycin extended to other viral families, including bunyaviruses (Rift Valley fever virus, Keystone virus), enteroviruses (coxsackievirus, rhinovirus), flavirivuses (Zika virus), and coronaviruses (human coronavirus 229E [HCoV-229E] and Middle East respiratory syndrome CoV [MERS-CoV]). In all viral infections, we observed significant reductions in virus titer in valinomycin-treated cells. In sum, we demonstrate the importance of potassium ions to virus infection, suggesting a potential therapeutic target to disrupt virus replication. Twit Text FOAVip Novel Ionophores Active against La Crosse Virus Identified through Rapid Antiviral Screening ????Antimicrob Agents Chemother http://www.kidney.de/pget.php?&tw=1&pmid=32284379 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32284379 #FOAvip English Wikipedia <ref>{{Cite pmid|32284379}}</ref> Novel Ionophores Active against La Crosse Virus Identified through Rapid Antiviral Screening #MMPMID32284379 Sandler ZJ; Firpo MR; Omoba OS; Vu MN; Menachery VD; Mounce BC Antimicrob Agents Chemother 2020[May]; 64 (6): ä PMID32284379show ga Bunyaviruses are significant human pathogens, causing diseases ranging from hemorrhagic fevers to encephalitis. Among these viruses, La Crosse virus (LACV), a member of the California serogroup, circulates in the eastern and midwestern United States. While LACV infection is often asymptomatic, dozens of cases of encephalitis are reported yearly. Unfortunately, no antivirals have been approved to treat LACV infection. Here, we developed a method to rapidly test potential antivirals against LACV infection. From this screen, we identified several potential antiviral molecules, including known antivirals. Additionally, we identified many novel antivirals that exhibited antiviral activity without affecting cellular viability. Valinomycin, a potassium ionophore, was among our top targets. We found that valinomycin exhibited potent anti-LACV activity in multiple cell types in a dose-dependent manner. Valinomycin did not affect particle stability or infectivity, suggesting that it may preclude virus replication by altering cellular potassium ions, a known determinant of LACV entry. We extended these results to other ionophores and found that the antiviral activity of valinomycin extended to other viral families, including bunyaviruses (Rift Valley fever virus, Keystone virus), enteroviruses (coxsackievirus, rhinovirus), flavirivuses (Zika virus), and coronaviruses (human coronavirus 229E [HCoV-229E] and Middle East respiratory syndrome CoV [MERS-CoV]). In all viral infections, we observed significant reductions in virus titer in valinomycin-treated cells. In sum, we demonstrate the importance of potassium ions to virus infection, suggesting a potential therapeutic target to disrupt virus replication. |Antiviral Agents/*pharmacology[MESH] |Coronavirus/drug effects[MESH] |Dose-Response Relationship, Drug[MESH] |Drug Evaluation, Preclinical[MESH] |Encephalitis, California/*drug therapy/virology[MESH] |Enterovirus/drug effects[MESH] |Flavivirus/drug effects[MESH] |Humans[MESH] |Ionophores/*pharmacology[MESH] |La Crosse virus/*drug effects[MESH] |Orthobunyavirus/drug effects[MESH] |Potassium/*metabolism[MESH] |United States[MESH] |Valinomycin/*pharmacology[MESH]Novel Ionophores Active against La Crosse Virus Identified through Rap(epmc).pdf DeepDyve Pubget Overpricing