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10.1038/s41374-020-0420-9 http://scihub22266oqcxt.onion/10.1038/s41374-020-0420-9 32238906!7111589!32238906     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Lab+Invest 2020 ; 100 (8): 1030-1041 Nephropedia Template TP {{tp|p=32238906|t=2020. Increased talin-vinculin spatial proximities in livers in response to spotted fever group rickettsial and Ebola virus infections |pdf=|usr=}}{{32238906}} gab.com Text Increased talin-vinculin spatial proximities in livers in response to spotted fever group rickettsial and Ebola virus infections JO: Lab Invest http://www.kidney.de/pget.php?&tw=1&pmid=32238906 #FOAvip Talin and vinculin, both actin-cytoskeleton-related proteins, have been documented to participate in establishing bacterial infections, respectively, as the adapter protein to mediate cytoskeleton-driven dynamics of the plasma membrane. However, little is known regarding the potential role of the talin-vinculin complex during spotted fever group rickettsial and Ebola virus infections, two dreadful infectious diseases in humans. Many functional properties of proteins are determined by their participation in protein-protein complexes, in a temporal and/or spatial manner. To resolve the limitation of application in using mouse primary antibodies on archival, multiple formalin-fixed mouse tissue samples, which were collected from experiments requiring high biocontainment, we developed a practical strategic proximity ligation assay (PLA) capable of employing one primary antibody raised in mouse to probe talin-vinculin spatial proximal complex in mouse tissue. We observed an increase of talin-vinculin spatial proximities in the livers of spotted fever Rickettsia australis or Ebola virus-infected mice when compared with mock mice. Furthermore, using EPAC1-knockout mice, we found that deletion of EPAC1 could suppress the formation of spatial proximal complex of talin-vinculin in rickettsial infections. In addition, we observed increased colocalization between spatial proximity of talin-vinculin and filamentous actin-specific phalloidin staining in single survival mouse from an ordinarily lethal dose of rickettsial or Ebola virus infection. These findings may help to delineate a fresh insight into the mechanisms underlying liver specific pathogenesis during infection with spotted fever rickettsia or Ebola virus in the mouse model. Twit Text FOAVip Increased talin-vinculin spatial proximities in livers in response to spotted fever group rickettsial and Ebola virus infections ????Lab Invest http://www.kidney.de/pget.php?&tw=1&pmid=32238906 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32238906 #FOAvip English Wikipedia <ref>{{Cite pmid|32238906}}</ref> Increased talin-vinculin spatial proximities in livers in response to spotted fever group rickettsial and Ebola virus infections #MMPMID32238906 Liu Y; Xiao J; Zhang B; Shelite TR; Su Z; Chang Q; Judy B; Li X; Drelich A; Bei J; Zhou Y; Zheng J; Jin Y; Rossi SL; Tang SJ; Wakamiya M; Saito T; Ksiazek T; Kaphalia B; Gong B Lab Invest 2020[Aug]; 100 (8): 1030-1041 PMID32238906show ga Talin and vinculin, both actin-cytoskeleton-related proteins, have been documented to participate in establishing bacterial infections, respectively, as the adapter protein to mediate cytoskeleton-driven dynamics of the plasma membrane. However, little is known regarding the potential role of the talin-vinculin complex during spotted fever group rickettsial and Ebola virus infections, two dreadful infectious diseases in humans. Many functional properties of proteins are determined by their participation in protein-protein complexes, in a temporal and/or spatial manner. To resolve the limitation of application in using mouse primary antibodies on archival, multiple formalin-fixed mouse tissue samples, which were collected from experiments requiring high biocontainment, we developed a practical strategic proximity ligation assay (PLA) capable of employing one primary antibody raised in mouse to probe talin-vinculin spatial proximal complex in mouse tissue. We observed an increase of talin-vinculin spatial proximities in the livers of spotted fever Rickettsia australis or Ebola virus-infected mice when compared with mock mice. Furthermore, using EPAC1-knockout mice, we found that deletion of EPAC1 could suppress the formation of spatial proximal complex of talin-vinculin in rickettsial infections. In addition, we observed increased colocalization between spatial proximity of talin-vinculin and filamentous actin-specific phalloidin staining in single survival mouse from an ordinarily lethal dose of rickettsial or Ebola virus infection. These findings may help to delineate a fresh insight into the mechanisms underlying liver specific pathogenesis during infection with spotted fever rickettsia or Ebola virus in the mouse model. |Actin Cytoskeleton/*metabolism[MESH] |Animals[MESH] |Cell Membrane/*metabolism[MESH] |Cells, Cultured[MESH] |Guanine Nucleotide Exchange Factors/genetics/metabolism[MESH] |Hemorrhagic Fever, Ebola/*metabolism[MESH] |Humans[MESH] |Liver/*metabolism/microbiology/virology[MESH] |Mice, Knockout[MESH] |Protein Binding[MESH] |Rickettsia/physiology[MESH] |Spotted Fever Group Rickettsiosis/metabolism/microbiology[MESH] |Talin/chemistry/*metabolism[MESH]Increased talin-vinculin spatial proximities in livers in response to (epmc).pdf DeepDyve Pubget Overpricing