Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1093/cid/ciaa345 http://scihub22266oqcxt.onion/10.1093/cid/ciaa345 32221523!7184442!32221523     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Clin+Infect+Dis 2020 ; 71 (15): 793-798 Nephropedia Template TP {{tp|p=32221523|t=2020. Quantitative Detection and Viral Load Analysis of SARS-CoV-2 in Infected Patients |pdf=|usr=}}{{32221523}} gab.com Text Quantitative Detection and Viral Load Analysis of SARS-CoV-2 in Infected Patients JO: Clin Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=32221523 #FOAvip BACKGROUND: Coronavirus disease 2019 (COVID-19) has become a public health emergency. The widely used reverse transcription-polymerase chain reaction (RT-PCR) method has limitations for clinical diagnosis and treatment. METHODS: A total of 323 samples from 76 COVID-19-confirmed patients were analyzed by droplet digital PCR (ddPCR) and RT-PCR based 2 target genes (ORF1ab and N). Nasal swabs, throat swabs, sputum, blood, and urine were collected. Clinical and imaging data were obtained for clinical staging. RESULTS: In 95 samples that tested positive by both methods, the cycle threshold (Ct) of RT-PCR was highly correlated with the copy number of ddPCR (ORF1ab gene, R2 = 0.83; N gene, R2 = 0.87). Four (4/161) negative and 41 (41/67) single-gene positive samples tested by RT-PCR were positive according to ddPCR with viral loads ranging from 11.1 to 123.2 copies/test. The viral load of respiratory samples was then compared and the average viral load in sputum (17 429 +/- 6920 copies/test) was found to be significantly higher than in throat swabs (2552 +/- 1965 copies/test, P < .001) and nasal swabs (651 +/- 501 copies/test, P < .001). Furthermore, the viral loads in the early and progressive stages were significantly higher than that in the recovery stage (46 800 +/- 17 272 vs 1252 +/- 1027, P < .001) analyzed by sputum samples. CONCLUSIONS: Quantitative monitoring of viral load in lower respiratory tract samples helps to evaluate disease progression, especially in cases of low viral load. Twit Text FOAVip Quantitative Detection and Viral Load Analysis of SARS-CoV-2 in Infected Patients ????Clin Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=32221523 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32221523 #FOAvip English Wikipedia <ref>{{Cite pmid|32221523}}</ref> Quantitative Detection and Viral Load Analysis of SARS-CoV-2 in Infected Patients #MMPMID32221523 Yu F; Yan L; Wang N; Yang S; Wang L; Tang Y; Gao G; Wang S; Ma C; Xie R; Wang F; Tan C; Zhu L; Guo Y; Zhang F Clin Infect Dis 2020[Jul]; 71 (15): 793-798 PMID32221523show ga BACKGROUND: Coronavirus disease 2019 (COVID-19) has become a public health emergency. The widely used reverse transcription-polymerase chain reaction (RT-PCR) method has limitations for clinical diagnosis and treatment. METHODS: A total of 323 samples from 76 COVID-19-confirmed patients were analyzed by droplet digital PCR (ddPCR) and RT-PCR based 2 target genes (ORF1ab and N). Nasal swabs, throat swabs, sputum, blood, and urine were collected. Clinical and imaging data were obtained for clinical staging. RESULTS: In 95 samples that tested positive by both methods, the cycle threshold (Ct) of RT-PCR was highly correlated with the copy number of ddPCR (ORF1ab gene, R2 = 0.83; N gene, R2 = 0.87). Four (4/161) negative and 41 (41/67) single-gene positive samples tested by RT-PCR were positive according to ddPCR with viral loads ranging from 11.1 to 123.2 copies/test. The viral load of respiratory samples was then compared and the average viral load in sputum (17 429 +/- 6920 copies/test) was found to be significantly higher than in throat swabs (2552 +/- 1965 copies/test, P < .001) and nasal swabs (651 +/- 501 copies/test, P < .001). Furthermore, the viral loads in the early and progressive stages were significantly higher than that in the recovery stage (46 800 +/- 17 272 vs 1252 +/- 1027, P < .001) analyzed by sputum samples. CONCLUSIONS: Quantitative monitoring of viral load in lower respiratory tract samples helps to evaluate disease progression, especially in cases of low viral load. |Adult[MESH] |Betacoronavirus/*genetics[MESH] |COVID-19[MESH] |Coronavirus Infections/*diagnosis/*virology[MESH] |Diagnostic Tests, Routine/methods[MESH] |False Negative Reactions[MESH] |Female[MESH] |Humans[MESH] |Male[MESH] |Middle Aged[MESH] |Pandemics[MESH] |Pneumonia, Viral/*diagnosis/*virology[MESH] |Real-Time Polymerase Chain Reaction/methods[MESH] |Respiratory System/virology[MESH] |SARS-CoV-2[MESH] |Serologic Tests/methods[MESH] |Sputum/virology[MESH]Quantitative Detection and Viral Load Analysis of SARS-CoV-2 in Infect(epmc).pdf DeepDyve Pubget Overpricing