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10.1016/j.msec.2020.110640 http://scihub22266oqcxt.onion/10.1016/j.msec.2020.110640 32204074!ä!32204074 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Mater+Sci+Eng+C+Mater+Biol+Appl 2020 ; 110 (ä): 110640 Nephropedia Template TP {{tp|p=32204074|t=2020. Hydrogen inhibits the osteoclastogenesis of mouse bone marrow mononuclear cells |pdf=|usr=}}{{32204074}} gab.com Text Hydrogen inhibits the osteoclastogenesis of mouse bone marrow mononuclear cells JO: Mater Sci Eng C Mater Biol Appl http://www.kidney.de/pget.php?&tw=1&pmid=32204074 #FOAvip Hydrogen (H(2)) is one of the major biodegradation products of magnesium (Mg) alloys implanted for bony fracture healing and reconstruction; H(2) thus plays a significant role in the regulation of local microenvironment and the biology of resident cells. The interactions between the H(2) and the local cells are of great interest, and a full understanding of the effect of H(2) on bone marrow mononuclear cells (BMMCs) would accelerate the development of effective strategies for successful bony healing. This study investigates how H(2), with different concentrations and durations, regulates the osteoclastogenesis of mouse BMMCs. First, using H(2) with five concentrations (0%, 2%, 25%, 50% and 75%) and three durations (5, 7 and 10 days), the osteoclastogenesis of mouse BMMCs in these H(2) conditions were measured using TRAP staining, F-actin ring formation assay, pit formation assay and RT-qPCR analysis. Based on these findings, the proliferation assay, apoptosis assay, western blot analysis and ELISA assay of BMMCs after osteoclast induction were performed. The findings showed that H(2) (especially the 50% and 75% H(2)) obviously inhibited the osteoclast formation, function and osteoclast-related genes expression of osteoclast-induced BMMCs; additionally, H(2) (50%) was found to reduce the proliferation, promote the apoptosis and inhibit the expression of osteoclast-related proteins of BMMCs with the presence of osteoclast-induced medium. Therefore, H(2) significantly inhibited the osteoclastogenesis of mouse BMMCs, which may become a new therapeutic agent for anti-bony resorption and open new avenues for the translational research of Mg alloys. Twit Text FOAVip Hydrogen inhibits the osteoclastogenesis of mouse bone marrow mononuclear cells ????Mater Sci Eng C Mater Biol Appl http://www.kidney.de/pget.php?&tw=1&pmid=32204074 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32204074 #FOAvip English Wikipedia <ref>{{Cite pmid|32204074}}</ref> Hydrogen inhibits the osteoclastogenesis of mouse bone marrow mononuclear cells #MMPMID32204074 Liu Y; Wang DL; Huang YC; Wang TB; Zeng H Mater Sci Eng C Mater Biol Appl 2020[May]; 110 (ä): 110640 PMID32204074show ga Hydrogen (H(2)) is one of the major biodegradation products of magnesium (Mg) alloys implanted for bony fracture healing and reconstruction; H(2) thus plays a significant role in the regulation of local microenvironment and the biology of resident cells. The interactions between the H(2) and the local cells are of great interest, and a full understanding of the effect of H(2) on bone marrow mononuclear cells (BMMCs) would accelerate the development of effective strategies for successful bony healing. This study investigates how H(2), with different concentrations and durations, regulates the osteoclastogenesis of mouse BMMCs. First, using H(2) with five concentrations (0%, 2%, 25%, 50% and 75%) and three durations (5, 7 and 10 days), the osteoclastogenesis of mouse BMMCs in these H(2) conditions were measured using TRAP staining, F-actin ring formation assay, pit formation assay and RT-qPCR analysis. Based on these findings, the proliferation assay, apoptosis assay, western blot analysis and ELISA assay of BMMCs after osteoclast induction were performed. The findings showed that H(2) (especially the 50% and 75% H(2)) obviously inhibited the osteoclast formation, function and osteoclast-related genes expression of osteoclast-induced BMMCs; additionally, H(2) (50%) was found to reduce the proliferation, promote the apoptosis and inhibit the expression of osteoclast-related proteins of BMMCs with the presence of osteoclast-induced medium. Therefore, H(2) significantly inhibited the osteoclastogenesis of mouse BMMCs, which may become a new therapeutic agent for anti-bony resorption and open new avenues for the translational research of Mg alloys. |Animals[MESH] |Apoptosis/drug effects[MESH] |Bone Marrow Cells/*cytology/drug effects/metabolism[MESH] |Cell Proliferation/drug effects[MESH] |Gene Expression Regulation/drug effects[MESH] |Hydrogen/*pharmacology[MESH] |Leukocytes, Mononuclear/*cytology/drug effects[MESH] |Mice, Inbred C57BL[MESH] |Osteoclasts/cytology/drug effects/metabolism[MESH]Hydrogen inhibits the osteoclastogenesis of mouse bone marrow mononucl(epmc).pdf DeepDyve Pubget Overpricing