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10.1080/22221751.2020.1738905 http://scihub22266oqcxt.onion/10.1080/22221751.2020.1738905 32174267!7144283!32174267     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=32174267&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       Emerg+Microbes+Infect 2020 ; 9 (1): 597-600 Nephropedia Template TP {{tp|p=32174267|t=2020. Era of molecular diagnosis for pathogen identification of unexplained pneumonia, lessons to be learned |pdf=|usr=}}{{32174267}} gab.com Text Era of molecular diagnosis for pathogen identification of unexplained pneumonia, lessons to be learned JO: Emerg Microbes Infect http://www.kidney.de/pget.php?&tw=1&pmid=32174267 #FOAvip Unexplained pneumonia (UP) caused by a novel coronavirus SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) emerged in China in late December 2019 and has infected more than 9000 cases by 31 January 2020. Shanghai reported the first imported case of COVID-19 (Coronavirus Disease 2019) in 20 January 2020. A combinative approach of real-time RT-PCR, CRISPR-based assay and metagenomic next-generation sequencing (mNGS) were used to diagnose this unexplained pneumonia patient. Real-time RT-PCR and CRISPR-based assay both reported positive. This sample belonged to Betacoronavirus and shared a more than 99% nucleotide (nt) identity with the Wuhan SARS-CoV-2 isolates. We further compared pros and cons of common molecular diagnostics in UP. In this study, we illustrated the importance of combining molecular diagnostics to rule out common pathogens and performed mNGS to obtain unbiased potential pathogen result for the diagnosis of UP. Twit Text FOAVip Era of molecular diagnosis for pathogen identification of unexplained pneumonia, lessons to be learned ????Emerg Microbes Infect http://www.kidney.de/pget.php?&tw=1&pmid=32174267 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32174267 #FOAvip English Wikipedia <ref>{{Cite pmid|32174267}}</ref> Era of molecular diagnosis for pathogen identification of unexplained pneumonia, lessons to be learned #MMPMID32174267 Ai JW; Zhang Y; Zhang HC; Xu T; Zhang WH Emerg Microbes Infect 2020[]; 9 (1): 597-600 PMID32174267show ga Unexplained pneumonia (UP) caused by a novel coronavirus SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) emerged in China in late December 2019 and has infected more than 9000 cases by 31 January 2020. Shanghai reported the first imported case of COVID-19 (Coronavirus Disease 2019) in 20 January 2020. A combinative approach of real-time RT-PCR, CRISPR-based assay and metagenomic next-generation sequencing (mNGS) were used to diagnose this unexplained pneumonia patient. Real-time RT-PCR and CRISPR-based assay both reported positive. This sample belonged to Betacoronavirus and shared a more than 99% nucleotide (nt) identity with the Wuhan SARS-CoV-2 isolates. We further compared pros and cons of common molecular diagnostics in UP. In this study, we illustrated the importance of combining molecular diagnostics to rule out common pathogens and performed mNGS to obtain unbiased potential pathogen result for the diagnosis of UP. |*Clinical Laboratory Techniques[MESH] |Betacoronavirus/genetics/isolation & purification[MESH] |COVID-19[MESH] |COVID-19 Testing[MESH] |COVID-19 Vaccines[MESH] |Coronavirus Infections/*diagnosis/epidemiology/transmission[MESH] |Humans[MESH] |Molecular Diagnostic Techniques[MESH] |Phylogeny[MESH] |Pneumonia, Viral/*diagnosis/epidemiology/transmission[MESH]Era of molecular diagnosis for pathogen identification of unexplained (epmc).pdf DeepDyve Pubget Overpricing