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10.1073/pnas.2002589117

http://scihub22266oqcxt.onion/10.1073/pnas.2002589117
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32165541!7132130!32165541
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suck abstract from ncbi


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pmid32165541      Proc+Natl+Acad+Sci+U+S+A 2020 ; 117 (13): 7001-7003
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  • Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells #MMPMID32165541
  • Matsuyama S; Nao N; Shirato K; Kawase M; Saito S; Takayama I; Nagata N; Sekizuka T; Katoh H; Kato F; Sakata M; Tahara M; Kutsuna S; Ohmagari N; Kuroda M; Suzuki T; Kageyama T; Takeda M
  • Proc Natl Acad Sci U S A 2020[Mar]; 117 (13): 7001-7003 PMID32165541show ga
  • A novel betacoronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which caused a large respiratory outbreak in Wuhan, China in December 2019, is currently spreading across many countries globally. Here, we show that a TMPRSS2-expressing VeroE6 cell line is highly susceptible to SARS-CoV-2 infection, making it useful for isolating and propagating SARS-CoV-2. Our results reveal that, in common with SARS- and Middle East respiratory syndrome-CoV, SARS-CoV-2 infection is enhanced by TMPRSS2.
  • |Animals[MESH]
  • |Betacoronavirus/*isolation & purification[MESH]
  • |COVID-19[MESH]
  • |Cell Line[MESH]
  • |Chlorocebus aethiops[MESH]
  • |Coronavirus Infections/*virology[MESH]
  • |Disease Outbreaks[MESH]
  • |Humans[MESH]
  • |Pandemics[MESH]
  • |Pneumonia, Viral/*virology[MESH]
  • |RNA, Viral/metabolism[MESH]
  • |SARS-CoV-2[MESH]
  • |Serine Endopeptidases/*metabolism[MESH]
  • |Vero Cells[MESH]


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