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10.3201/eid2606.200516 http://scihub22266oqcxt.onion/10.3201/eid2606.200516 32160149!7258473!32160149     free     free     free Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 235.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Emerg+Infect+Dis 2020 ; 26 (6): 1266-1273 Nephropedia Template TP {{tp|p=32160149|t=2020. Severe Acute Respiratory Syndrome Coronavirus 2 from Patient with Coronavirus Disease, United States |pdf=|usr=}}{{32160149}} gab.com Text Severe Acute Respiratory Syndrome Coronavirus 2 from Patient with Coronavirus Disease, United States JO: Emerg Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=32160149 #FOAvip The etiologic agent of an outbreak of pneumonia in Wuhan, China, was identified as severe acute respiratory syndrome coronavirus 2 in January 2020. A patient in the United States was given a diagnosis of infection with this virus by the state of Washington and the US Centers for Disease Control and Prevention on January 20, 2020. We isolated virus from nasopharyngeal and oropharyngeal specimens from this patient and characterized the viral sequence, replication properties, and cell culture tropism. We found that the virus replicates to high titer in Vero-CCL81 cells and Vero E6 cells in the absence of trypsin. We also deposited the virus into 2 virus repositories, making it broadly available to the public health and research communities. We hope that open access to this reagent will expedite development of medical countermeasures. Twit Text FOAVip Severe Acute Respiratory Syndrome Coronavirus 2 from Patient with Coronavirus Disease, United States ????Emerg Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=32160149 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32160149 #FOAvip English Wikipedia <ref>{{Cite pmid|32160149}}</ref> Severe Acute Respiratory Syndrome Coronavirus 2 from Patient with Coronavirus Disease, United States #MMPMID32160149 Harcourt J; Tamin A; Lu X; Kamili S; Sakthivel SK; Murray J; Queen K; Tao Y; Paden CR; Zhang J; Li Y; Uehara A; Wang H; Goldsmith C; Bullock HA; Wang L; Whitaker B; Lynch B; Gautam R; Schindewolf C; Lokugamage KG; Scharton D; Plante JA; Mirchandani D; Widen SG; Narayanan K; Makino S; Ksiazek TG; Plante KS; Weaver SC; Lindstrom S; Tong S; Menachery VD; Thornburg NJ Emerg Infect Dis 2020[Jun]; 26 (6): 1266-1273 PMID32160149show ga The etiologic agent of an outbreak of pneumonia in Wuhan, China, was identified as severe acute respiratory syndrome coronavirus 2 in January 2020. A patient in the United States was given a diagnosis of infection with this virus by the state of Washington and the US Centers for Disease Control and Prevention on January 20, 2020. We isolated virus from nasopharyngeal and oropharyngeal specimens from this patient and characterized the viral sequence, replication properties, and cell culture tropism. We found that the virus replicates to high titer in Vero-CCL81 cells and Vero E6 cells in the absence of trypsin. We also deposited the virus into 2 virus repositories, making it broadly available to the public health and research communities. We hope that open access to this reagent will expedite development of medical countermeasures. |Animals[MESH] |Betacoronavirus/genetics/*isolation & purification/physiology[MESH] |COVID-19[MESH] |Cell Line[MESH] |Chlorocebus aethiops[MESH] |Coronavirus Infections/*diagnosis[MESH] |Genome, Viral[MESH] |Humans[MESH] |Nasopharynx/virology[MESH] |Oropharynx/virology[MESH] |Pandemics[MESH] |Pneumonia, Viral/*diagnosis[MESH] |SARS-CoV-2[MESH] |Vero Cells[MESH] |Viral Tropism[MESH] |Virus Replication[MESH]Severe Acute Respiratory Syndrome Coronavirus 2 from Patient with Coro(epmc).pdf DeepDyve Pubget Overpricing