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10.24171/j.phrp.2020.11.1.02

http://scihub22266oqcxt.onion/10.24171/j.phrp.2020.11.1.02
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32149036!7045880!32149036
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suck abstract from ncbi


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pmid32149036      Osong+Public+Health+Res+Perspect 2020 ; 11 (1): 3-7
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  • Identification of Coronavirus Isolated from a Patient in Korea with COVID-19 #MMPMID32149036
  • Kim JM; Chung YS; Jo HJ; Lee NJ; Kim MS; Woo SH; Park S; Kim JW; Kim HM; Han MG
  • Osong Public Health Res Perspect 2020[Feb]; 11 (1): 3-7 PMID32149036show ga
  • OBJECTIVES: Following reports of patients with unexplained pneumonia at the end of December 2019 in Wuhan, China, the causative agent was identified as coronavirus (SARS-CoV-2), and the 2019 novel coronavirus disease was named COVID-19 by the World Health Organization. Putative patients with COVID-19 have been identified in South Korea, and attempts have been made to isolate the pathogen from these patients. METHODS: Upper and lower respiratory tract secretion samples from putative patients with COVID-19 were inoculated onto cells to isolate the virus. Full genome sequencing and electron microscopy were used to identify the virus. RESULTS: The virus replicated in Vero cells and cytopathic effects were observed. Full genome sequencing showed that the virus genome exhibited sequence homology of more than 99.9% with SARS-CoV-2 which was isolated from patients from other countries, for instance China. Sequence homology of SARS-CoV-2 with SARS-CoV, and MERS-CoV was 77.5% and 50%, respectively. Coronavirus-specific morphology was observed by electron microscopy in virus-infected Vero cells. CONCLUSION: SARS-CoV-2 was isolated from putative patients with unexplained pneumonia and intermittent coughing and fever. The isolated virus was named BetaCoV/Korea/KCDC03/2020.
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