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10.1021/acsami.9b23591 http://scihub22266oqcxt.onion/10.1021/acsami.9b23591 32101396!ä!32101396 Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=32101396&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       ACS+Appl+Mater+Interfaces 2020 ; 12 (11): 12533-12540 Nephropedia Template TP {{tp|p=32101396|t=2020. Nanoplasmonic On-Chip PCR for Rapid Precision Molecular Diagnostics |pdf=|usr=}}{{32101396}} gab.com Text Nanoplasmonic On-Chip PCR for Rapid Precision Molecular Diagnostics JO: ACS Appl Mater Interfaces http://www.kidney.de/pget.php?&tw=1&pmid=32101396 #FOAvip Emerging molecular diagnosis requires ultrafast polymerase chain reaction (PCR) on chip for rapid precise detection of infectious diseases in the point-of-care test. Here, we report nanoplasmonic on-chip PCR for rapid precision molecular diagnostics. The nanoplasmonic pillar arrays (NPA) comprise gold nanoislands on the top and sidewall of large-scale glass nanopillar arrays. The nanoplasmonic pillars enhance light absorption of a white light-emitting diode (LED) over the whole visible range due to strong electromagnetic hotspots between the nanoislands. As a result, they effectively induce photothermal heating for ultrafast PCR thermal cycling. The temperature profile of NPA exhibits 30 cycles between 98 and 60 degrees C for a total of 3 min and 30 s during the cyclic excitation of white LED light. The experimental results also demonstrate the rapid DNA amplification of both 0.1 ng muL(-1) of lambda-DNA in 20 thermal cycles and 0.1 ng muL(-1) of complementary DNA of Middle East respiratory syndrome coronavirus in 30 thermal cycles using a conventional PCR volume of 15 muL. This nanoplasmonic PCR technique provides a new opportunity for rapid precision molecular diagnostics. Twit Text FOAVip Nanoplasmonic On-Chip PCR for Rapid Precision Molecular Diagnostics ????ACS Appl Mater Interfaces http://www.kidney.de/pget.php?&tw=1&pmid=32101396 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32101396 #FOAvip English Wikipedia <ref>{{Cite pmid|32101396}}</ref> Nanoplasmonic On-Chip PCR for Rapid Precision Molecular Diagnostics #MMPMID32101396 Lee Y; Kang BH; Kang M; Chung DR; Yi GS; Lee LP; Jeong KH ACS Appl Mater Interfaces 2020[Mar]; 12 (11): 12533-12540 PMID32101396show ga Emerging molecular diagnosis requires ultrafast polymerase chain reaction (PCR) on chip for rapid precise detection of infectious diseases in the point-of-care test. Here, we report nanoplasmonic on-chip PCR for rapid precision molecular diagnostics. The nanoplasmonic pillar arrays (NPA) comprise gold nanoislands on the top and sidewall of large-scale glass nanopillar arrays. The nanoplasmonic pillars enhance light absorption of a white light-emitting diode (LED) over the whole visible range due to strong electromagnetic hotspots between the nanoislands. As a result, they effectively induce photothermal heating for ultrafast PCR thermal cycling. The temperature profile of NPA exhibits 30 cycles between 98 and 60 degrees C for a total of 3 min and 30 s during the cyclic excitation of white LED light. The experimental results also demonstrate the rapid DNA amplification of both 0.1 ng muL(-1) of lambda-DNA in 20 thermal cycles and 0.1 ng muL(-1) of complementary DNA of Middle East respiratory syndrome coronavirus in 30 thermal cycles using a conventional PCR volume of 15 muL. This nanoplasmonic PCR technique provides a new opportunity for rapid precision molecular diagnostics. |Communicable Diseases/*diagnosis/genetics[MESH] |DNA/*analysis/metabolism[MESH] |Gold/chemistry[MESH] |Humans[MESH] |Lab-On-A-Chip Devices[MESH] |Light[MESH] |Nanofibers/*chemistry[MESH] |Oligonucleotide Array Sequence Analysis[MESH] |Point-of-Care Systems[MESH] |Polymerase Chain Reaction/instrumentation/*methods[MESH]Nanoplasmonic On-Chip PCR for Rapid Precision Molecular Diagnostics (epmc).pdf DeepDyve Pubget Overpricing