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10.3390/v12020240 http://scihub22266oqcxt.onion/10.3390/v12020240 32098094!7077318!32098094     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 251.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Viruses 2020 ; 12 (2): ä Nephropedia Template TP {{tp|p=32098094|t=2020. Structural Basis for Inhibiting Porcine Epidemic Diarrhea Virus Replication with the 3C-Like Protease Inhibitor GC376 |pdf=|usr=}}{{32098094}} gab.com Text Structural Basis for Inhibiting Porcine Epidemic Diarrhea Virus Replication with the 3C-Like Protease Inhibitor GC376 JO: Viruses http://www.kidney.de/pget.php?&tw=1&pmid=32098094 #FOAvip Porcine epidemic diarrhea virus (PEDV), being highly virulent and contagious in piglets, has caused significant damage to the pork industries of many countries worldwide. There are no commercial drugs targeting coronaviruses (CoVs), and few studies on anti-PEDV inhibitors. The coronavirus 3C-like protease (3CL(pro)) has a conserved structure and catalytic mechanism and plays a key role during viral polyprotein processing, thus serving as an appealing antiviral drug target. Here, we report the anti-PEDV effect of the broad-spectrum inhibitor GC376 (targeting 3Cpro or 3CL(pro) of viruses in the picornavirus-like supercluster). GC376 was highly effective against the PEDV 3CL(pro) and exerted similar inhibitory effects on two PEDV strains. Furthermore, the structure of the PEDV 3CL(pro) in complex with GC376 was determined at 1.65 A. We elucidated structural details and analyzed the differences between GC376 binding with the PEDV 3CL(pro) and GC376 binding with the transmissible gastroenteritis virus (TGEV) 3CL(pro). Finally, we explored the substrate specificity of PEDV 3CL(pro) at the P2 site and analyzed the effects of Leu group modification in GC376 on inhibiting PEDV infection. This study helps us to understand better the PEDV 3CL(pro) substrate specificity, providing information on the optimization of GC376 for development as an antiviral therapeutic against coronaviruses. Twit Text FOAVip Structural Basis for Inhibiting Porcine Epidemic Diarrhea Virus Replication with the 3C-Like Protease Inhibitor GC376 ????Viruses http://www.kidney.de/pget.php?&tw=1&pmid=32098094 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32098094 #FOAvip English Wikipedia <ref>{{Cite pmid|32098094}}</ref> Structural Basis for Inhibiting Porcine Epidemic Diarrhea Virus Replication with the 3C-Like Protease Inhibitor GC376 #MMPMID32098094 Ye G; Wang X; Tong X; Shi Y; Fu ZF; Peng G Viruses 2020[Feb]; 12 (2): ä PMID32098094show ga Porcine epidemic diarrhea virus (PEDV), being highly virulent and contagious in piglets, has caused significant damage to the pork industries of many countries worldwide. There are no commercial drugs targeting coronaviruses (CoVs), and few studies on anti-PEDV inhibitors. The coronavirus 3C-like protease (3CL(pro)) has a conserved structure and catalytic mechanism and plays a key role during viral polyprotein processing, thus serving as an appealing antiviral drug target. Here, we report the anti-PEDV effect of the broad-spectrum inhibitor GC376 (targeting 3Cpro or 3CL(pro) of viruses in the picornavirus-like supercluster). GC376 was highly effective against the PEDV 3CL(pro) and exerted similar inhibitory effects on two PEDV strains. Furthermore, the structure of the PEDV 3CL(pro) in complex with GC376 was determined at 1.65 A. We elucidated structural details and analyzed the differences between GC376 binding with the PEDV 3CL(pro) and GC376 binding with the transmissible gastroenteritis virus (TGEV) 3CL(pro). Finally, we explored the substrate specificity of PEDV 3CL(pro) at the P2 site and analyzed the effects of Leu group modification in GC376 on inhibiting PEDV infection. This study helps us to understand better the PEDV 3CL(pro) substrate specificity, providing information on the optimization of GC376 for development as an antiviral therapeutic against coronaviruses. |Animals[MESH] |Antiviral Agents/chemistry/metabolism/*pharmacology[MESH] |Catalytic Domain[MESH] |Chlorocebus aethiops[MESH] |Crystallography, X-Ray[MESH] |Models, Molecular[MESH] |Peptide Hydrolases/*chemistry/metabolism[MESH] |Porcine epidemic diarrhea virus/*drug effects/enzymology/physiology[MESH] |Protease Inhibitors/chemistry/metabolism/*pharmacology[MESH] |Pyrrolidines/chemistry/metabolism/*pharmacology[MESH] |Substrate Specificity[MESH] |Sulfonic Acids[MESH] |Transmissible gastroenteritis virus/enzymology[MESH] |Vero Cells[MESH]Structural Basis for Inhibiting Porcine Epidemic Diarrhea Virus Replic(epmc).pdf DeepDyve Pubget Overpricing