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10.1080/22221751.2020.1730245 http://scihub22266oqcxt.onion/10.1080/22221751.2020.1730245 32090689!7054919!32090689     free     free     free Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 215.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Emerg+Microbes+Infect 2020 ; 9 (1): 457-468 Nephropedia Template TP {{tp|p=32090689|t=2020. Trypsin promotes porcine deltacoronavirus mediating cell-to-cell fusion in a cell type-dependent manner |pdf=|usr=}}{{32090689}} gab.com Text Trypsin promotes porcine deltacoronavirus mediating cell-to-cell fusion in a cell type-dependent manner JO: Emerg Microbes Infect http://www.kidney.de/pget.php?&tw=1&pmid=32090689 #FOAvip Porcine deltacoronavirus (PDCoV) is a newly emerging threat to the global porcine industry. PDCoV has been successfully isolated using various medium additives including trypsin, and although we know it is important for viral replication, the mechanism has not been fully elucidated. Here, we systematically investigated the role of trypsin in PDCoV replication including cell entry, cell-to-cell membrane fusion and virus release. Using pseudovirus entry assays, we demonstrated that PDCoV entry is not trypsin dependent. Furthermore, unlike porcine epidemic diarrhea virus (PEDV), in which trypsin is important for the release of virus from infected cells, PDCoV release was not affected by trypsin. We also demonstrated that trypsin promotes PDCoV replication by enhancing cell-to-cell membrane fusion. Most importantly, our study illustrates two distinct spreading patterns from infected cells to uninfected cells during PDCoV transmission, and the role of trypsin in PDCoV replication in cells with different virus spreading types. Overall, these results clarify that trypsin promotes PDCoV replication by mediating cell-to-cell fusion transmission but is not crucial for viral entry. This knowledge can potentially contribute to improvement of virus production efficiency in culture, not only for vaccine preparation but also to develop antiviral treatments. Twit Text FOAVip Trypsin promotes porcine deltacoronavirus mediating cell-to-cell fusion in a cell type-dependent manner ????Emerg Microbes Infect http://www.kidney.de/pget.php?&tw=1&pmid=32090689 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32090689 #FOAvip English Wikipedia <ref>{{Cite pmid|32090689}}</ref> Trypsin promotes porcine deltacoronavirus mediating cell-to-cell fusion in a cell type-dependent manner #MMPMID32090689 Yang YL; Meng F; Qin P; Herrler G; Huang YW; Tang YD Emerg Microbes Infect 2020[]; 9 (1): 457-468 PMID32090689show ga Porcine deltacoronavirus (PDCoV) is a newly emerging threat to the global porcine industry. PDCoV has been successfully isolated using various medium additives including trypsin, and although we know it is important for viral replication, the mechanism has not been fully elucidated. Here, we systematically investigated the role of trypsin in PDCoV replication including cell entry, cell-to-cell membrane fusion and virus release. Using pseudovirus entry assays, we demonstrated that PDCoV entry is not trypsin dependent. Furthermore, unlike porcine epidemic diarrhea virus (PEDV), in which trypsin is important for the release of virus from infected cells, PDCoV release was not affected by trypsin. We also demonstrated that trypsin promotes PDCoV replication by enhancing cell-to-cell membrane fusion. Most importantly, our study illustrates two distinct spreading patterns from infected cells to uninfected cells during PDCoV transmission, and the role of trypsin in PDCoV replication in cells with different virus spreading types. Overall, these results clarify that trypsin promotes PDCoV replication by mediating cell-to-cell fusion transmission but is not crucial for viral entry. This knowledge can potentially contribute to improvement of virus production efficiency in culture, not only for vaccine preparation but also to develop antiviral treatments. |*Cell Fusion[MESH] |*Membrane Fusion[MESH] |Animals[MESH] |Cell Line[MESH] |Coronavirus/*physiology[MESH] |Humans[MESH] |Swine[MESH] |Trypsin/*metabolism[MESH] |Virus Internalization[MESH]Trypsin promotes porcine deltacoronavirus mediating cell-to-cell fusio(epmc).pdf DeepDyve Pubget Overpricing