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10.1002/jmv.25719 http://scihub22266oqcxt.onion/10.1002/jmv.25719 32083328!7228367!32083328     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Med+Virol 2020 ; 92 (6): 584-588 Nephropedia Template TP {{tp|p=32083328|t=2020. COVID-2019: The role of the nsp2 and nsp3 in its pathogenesis |pdf=|usr=}}{{32083328}} gab.com Text COVID-2019: The role of the nsp2 and nsp3 in its pathogenesis JO: J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=32083328 #FOAvip Last December 2019, a new virus, named novel Coronavirus (COVID-2019) causing many cases of severe pneumonia was reported in Wuhan, China. The virus knowledge is limited and especially about COVID-2019 pathogenesis. The Open Reading Frame 1ab (ORF1ab) of COVID-2019 has been analyzed to evidence the presence of mutation caused by selective pressure on the virus. For selective pressure analysis fast-unconstrained Bayesian approximation (FUBAR) was used. Homology modelling has been performed by SwissModel and HHPred servers. The presence of transmembrane helical segments in Coronavirus ORF1ab non structural protein 2 (nsp2) and nsp3 was tested by TMHMM, MEMSAT, and MEMPACK tools. Three-dimensional structures have been analyzed and displayed using PyMOL. FUBAR analysis revealed the presence of potential sites under positive selective pressure (P < .05). Position 723 in the COVID-2019 has a serine instead a glycine residue, while at aminoacidic position 1010 a proline instead an isoleucine. Significant (P < .05) pervasive negative selection in 2416 sites (55%) was found. The positive selective pressure could account for some clinical features of this virus compared with severe acute respiratory syndrome (SARS) and Bat SARS-like CoV. The stabilizing mutation falling in the endosome-associated-protein-like domain of the nsp2 protein could account for COVID-2019 high ability of contagious, while the destabilizing mutation in nsp3 proteins could suggest a potential mechanism differentiating COVID-2019 from SARS. These data could be helpful for further investigation aimed to identify potential therapeutic targets or vaccine strategy, especially in the actual moment when the epidemic is ongoing and the scientific community is trying to enrich knowledge about this new viral pathogen. Twit Text FOAVip COVID-2019: The role of the nsp2 and nsp3 in its pathogenesis ????J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=32083328 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32083328 #FOAvip English Wikipedia <ref>{{Cite pmid|32083328}}</ref> COVID-2019: The role of the nsp2 and nsp3 in its pathogenesis #MMPMID32083328 Angeletti S; Benvenuto D; Bianchi M; Giovanetti M; Pascarella S; Ciccozzi M J Med Virol 2020[Jun]; 92 (6): 584-588 PMID32083328show ga Last December 2019, a new virus, named novel Coronavirus (COVID-2019) causing many cases of severe pneumonia was reported in Wuhan, China. The virus knowledge is limited and especially about COVID-2019 pathogenesis. The Open Reading Frame 1ab (ORF1ab) of COVID-2019 has been analyzed to evidence the presence of mutation caused by selective pressure on the virus. For selective pressure analysis fast-unconstrained Bayesian approximation (FUBAR) was used. Homology modelling has been performed by SwissModel and HHPred servers. The presence of transmembrane helical segments in Coronavirus ORF1ab non structural protein 2 (nsp2) and nsp3 was tested by TMHMM, MEMSAT, and MEMPACK tools. Three-dimensional structures have been analyzed and displayed using PyMOL. FUBAR analysis revealed the presence of potential sites under positive selective pressure (P < .05). Position 723 in the COVID-2019 has a serine instead a glycine residue, while at aminoacidic position 1010 a proline instead an isoleucine. Significant (P < .05) pervasive negative selection in 2416 sites (55%) was found. The positive selective pressure could account for some clinical features of this virus compared with severe acute respiratory syndrome (SARS) and Bat SARS-like CoV. The stabilizing mutation falling in the endosome-associated-protein-like domain of the nsp2 protein could account for COVID-2019 high ability of contagious, while the destabilizing mutation in nsp3 proteins could suggest a potential mechanism differentiating COVID-2019 from SARS. These data could be helpful for further investigation aimed to identify potential therapeutic targets or vaccine strategy, especially in the actual moment when the epidemic is ongoing and the scientific community is trying to enrich knowledge about this new viral pathogen. |Betacoronavirus/*genetics/pathogenicity[MESH] |COVID-19[MESH] |Coronavirus Infections/virology[MESH] |Female[MESH] |Gene Expression[MESH] |Humans[MESH] |Male[MESH] |Models, Molecular[MESH] |Mutation[MESH] |Pandemics[MESH] |Pneumonia, Viral/virology[MESH] |Polyproteins[MESH] |Protein Conformation, alpha-Helical[MESH] |Protein Conformation, beta-Strand[MESH] |Protein Interaction Domains and Motifs[MESH] |SARS-CoV-2[MESH] |Selection, Genetic[MESH] |Severe acute respiratory syndrome-related coronavirus/*genetics/pathogenicity[MESH] |Structural Homology, Protein[MESH] |Viral Nonstructural Proteins/*chemistry/genetics/metabolism[MESH]COVID-2019: The role of the nsp2 and nsp3 in its pathogenesis (epmc).pdf DeepDyve Pubget Overpricing