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10.1074/jbc.RA119.011789 http://scihub22266oqcxt.onion/10.1074/jbc.RA119.011789 32079677!7152764!32079677     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Biol+Chem 2020 ; 295 (15): 4950-4962 Nephropedia Template TP {{tp|p=32079677|t=2020. Paraoxonase 3 functions as a chaperone to decrease functional expression of the epithelial sodium channel |pdf=|usr=}}{{32079677}} gab.com Text Paraoxonase 3 functions as a chaperone to decrease functional expression of the epithelial sodium channel JO: J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=32079677 #FOAvip The paraoxonase (PON) family comprises three highly conserved members: PON1, PON2, and PON3. They are orthologs of Caenorhabditis elegans MEC-6, an endoplasmic reticulum-resident chaperone that has a critical role in proper assembly and surface expression of the touch-sensing degenerin channel in nematodes. We have shown recently that MEC-6 and PON2 negatively regulate functional expression of the epithelial Na(+) channel (ENaC), suggesting that the chaperone function is conserved within this family. We hypothesized that other PON family members also modulate ion channel expression. Pon3 is specifically expressed in the aldosterone-sensitive distal tubules in the mouse kidney. We found here that knocking down endogenous Pon3 in mouse cortical collecting duct cells enhanced Na(+) transport, which was associated with increased gammaENaC abundance. We further examined Pon3 regulation of ENaC in two heterologous expression systems, Fisher rat thyroid cells and Xenopus oocytes. Pon3 coimmunoprecipitated with each of the three ENaC subunits in Fisher rat thyroid cells. As a result of this interaction, the whole-cell and surface abundance of ENaC alpha and gamma subunits was reduced by Pon3. When expressed in oocytes, Pon3 inhibited ENaC-mediated amiloride-sensitive Na(+) currents, in part by reducing the surface expression of ENaC. In contrast, Pon3 did not alter the response of ENaC to chymotrypsin-mediated proteolytic activation or [2-(trimethylammonium)ethyl]methanethiosulfonate-induced activation of alphabeta(S518C)gamma, suggesting that Pon3 does not affect channel open probability. Together, our results suggest that PON3 regulates ENaC expression by inhibiting its biogenesis and/or trafficking. Twit Text FOAVip Paraoxonase 3 functions as a chaperone to decrease functional expression of the epithelial sodium channel ????J Biol Chem http://www.kidney.de/pget.php?&tw=1&pmid=32079677 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32079677 #FOAvip English Wikipedia <ref>{{Cite pmid|32079677}}</ref> Paraoxonase 3 functions as a chaperone to decrease functional expression of the epithelial sodium channel #MMPMID32079677 Shi S; Montalbetti N; Wang X; Rush BM; Marciszyn AL; Baty CJ; Tan RJ; Carattino MD; Kleyman TR J Biol Chem 2020[Apr]; 295 (15): 4950-4962 PMID32079677show ga The paraoxonase (PON) family comprises three highly conserved members: PON1, PON2, and PON3. They are orthologs of Caenorhabditis elegans MEC-6, an endoplasmic reticulum-resident chaperone that has a critical role in proper assembly and surface expression of the touch-sensing degenerin channel in nematodes. We have shown recently that MEC-6 and PON2 negatively regulate functional expression of the epithelial Na(+) channel (ENaC), suggesting that the chaperone function is conserved within this family. We hypothesized that other PON family members also modulate ion channel expression. Pon3 is specifically expressed in the aldosterone-sensitive distal tubules in the mouse kidney. We found here that knocking down endogenous Pon3 in mouse cortical collecting duct cells enhanced Na(+) transport, which was associated with increased gammaENaC abundance. We further examined Pon3 regulation of ENaC in two heterologous expression systems, Fisher rat thyroid cells and Xenopus oocytes. Pon3 coimmunoprecipitated with each of the three ENaC subunits in Fisher rat thyroid cells. As a result of this interaction, the whole-cell and surface abundance of ENaC alpha and gamma subunits was reduced by Pon3. When expressed in oocytes, Pon3 inhibited ENaC-mediated amiloride-sensitive Na(+) currents, in part by reducing the surface expression of ENaC. In contrast, Pon3 did not alter the response of ENaC to chymotrypsin-mediated proteolytic activation or [2-(trimethylammonium)ethyl]methanethiosulfonate-induced activation of alphabeta(S518C)gamma, suggesting that Pon3 does not affect channel open probability. Together, our results suggest that PON3 regulates ENaC expression by inhibiting its biogenesis and/or trafficking. |Animals[MESH] |Aryldialkylphosphatase/genetics/*metabolism[MESH] |Cell Membrane/*metabolism[MESH] |Epithelial Sodium Channels/genetics/*metabolism[MESH] |Ion Transport[MESH] |Mice[MESH] |Molecular Chaperones[MESH] |Oocytes/cytology/*metabolism[MESH] |Rats[MESH] |Signal Transduction[MESH] |Sodium/*metabolism[MESH] |Thyroid Gland/cytology/*metabolism[MESH]Paraoxonase 3 functions as a chaperone to decrease functional expressi(epmc).pdf DeepDyve Pubget Overpricing