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10.1016/j.healun.2019.12.009 http://scihub22266oqcxt.onion/10.1016/j.healun.2019.12.009 32033844!7102671!32033844     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Heart+Lung+Transplant 2020 ; 39 (4): 379-388 Nephropedia Template TP {{tp|p=32033844|t=2020. Respiratory viral infection in lung transplantation induces exosomes that trigger chronic rejection |pdf=|usr=}}{{32033844}} gab.com Text Respiratory viral infection in lung transplantation induces exosomes that trigger chronic rejection JO: J Heart Lung Transplant http://www.kidney.de/pget.php?&tw=1&pmid=32033844 #FOAvip BACKGROUND: Respiratory viral infections can increase the risk of chronic lung allograft dysfunction after lung transplantation, but the mechanisms are unknown. In this study, we determined whether symptomatic respiratory viral infections after lung transplantation induce circulating exosomes that contain lung-associated self-antigens and assessed whether these exosomes activate immune responses to self-antigens. METHODS: Serum samples were collected from lung transplant recipients with symptomatic lower- and upper-tract respiratory viral infections and from non-symptomatic stable recipients. Exosomes were isolated via ultracentrifugation; purity was determined using sucrose cushion; and presence of lung self-antigens, 20S proteasome, and viral antigens for rhinovirus, coronavirus, and respiratory syncytial virus were determined using immunoblot. Mice were immunized with circulating exosomes from each group and resulting differential immune responses and lung histology were analyzed. RESULTS: Exosomes containing self-antigens, 20S proteasome, and viral antigens were detected at significantly higher levels (p < 0.05) in serum of recipients with symptomatic respiratory viral infections (n?=?35) as compared with stable controls (n?=?32). Mice immunized with exosomes from recipients with respiratory viral infections developed immune responses to self-antigens, fibrosis, small airway occlusion, and significant cellular infiltration; mice immunized with exosomes from controls did not (p < 0.05). CONCLUSIONS: Circulating exosomes isolated from lung transplant recipients diagnosed with respiratory viral infections contained lung self-antigens, viral antigens, and 20S proteasome and elicited immune responses to lung self-antigens that resulted in development of chronic lung allograft dysfunction in immunized mice. Twit Text FOAVip Respiratory viral infection in lung transplantation induces exosomes that trigger chronic rejection ????J Heart Lung Transplant http://www.kidney.de/pget.php?&tw=1&pmid=32033844 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32033844 #FOAvip English Wikipedia <ref>{{Cite pmid|32033844}}</ref> Respiratory viral infection in lung transplantation induces exosomes that trigger chronic rejection #MMPMID32033844 Gunasekaran M; Bansal S; Ravichandran R; Sharma M; Perincheri S; Rodriguez F; Hachem R; Fisher CE; Limaye AP; Omar A; Smith MA; Bremner RM; Mohanakumar T J Heart Lung Transplant 2020[Apr]; 39 (4): 379-388 PMID32033844show ga BACKGROUND: Respiratory viral infections can increase the risk of chronic lung allograft dysfunction after lung transplantation, but the mechanisms are unknown. In this study, we determined whether symptomatic respiratory viral infections after lung transplantation induce circulating exosomes that contain lung-associated self-antigens and assessed whether these exosomes activate immune responses to self-antigens. METHODS: Serum samples were collected from lung transplant recipients with symptomatic lower- and upper-tract respiratory viral infections and from non-symptomatic stable recipients. Exosomes were isolated via ultracentrifugation; purity was determined using sucrose cushion; and presence of lung self-antigens, 20S proteasome, and viral antigens for rhinovirus, coronavirus, and respiratory syncytial virus were determined using immunoblot. Mice were immunized with circulating exosomes from each group and resulting differential immune responses and lung histology were analyzed. RESULTS: Exosomes containing self-antigens, 20S proteasome, and viral antigens were detected at significantly higher levels (p < 0.05) in serum of recipients with symptomatic respiratory viral infections (n?=?35) as compared with stable controls (n?=?32). Mice immunized with exosomes from recipients with respiratory viral infections developed immune responses to self-antigens, fibrosis, small airway occlusion, and significant cellular infiltration; mice immunized with exosomes from controls did not (p < 0.05). CONCLUSIONS: Circulating exosomes isolated from lung transplant recipients diagnosed with respiratory viral infections contained lung self-antigens, viral antigens, and 20S proteasome and elicited immune responses to lung self-antigens that resulted in development of chronic lung allograft dysfunction in immunized mice. |Aged[MESH] |Animals[MESH] |Antigens, Viral/metabolism[MESH] |Autoantigens/metabolism[MESH] |Case-Control Studies[MESH] |Exosomes/*metabolism[MESH] |Female[MESH] |Graft Rejection/*etiology/*metabolism[MESH] |HLA Antigens/metabolism[MESH] |Humans[MESH] |Lung Transplantation/*adverse effects[MESH] |Male[MESH] |Mice[MESH] |Mice, Inbred C57BL[MESH] |Middle Aged[MESH] |Proteasome Endopeptidase Complex/metabolism[MESH] |Respiratory Tract Infections/complications/*metabolism/virology[MESH]Respiratory viral infection in lung transplantation induces exosomes t(epmc).pdf DeepDyve Pubget Overpricing