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10.1016/j.molimm.2020.01.020 http://scihub22266oqcxt.onion/10.1016/j.molimm.2020.01.020 32014632!7112685!32014632     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Mol+Immunol 2020 ; 119 (ä): 132-143 Nephropedia Template TP {{tp|p=32014632|t=2020. Camelid type I interferons: Identification and functional characterization of interferon alpha from the dromedary camel (Camelus dromedarius) |pdf=|usr=}}{{32014632}} gab.com Text Camelid type I interferons: Identification and functional characterization of interferon alpha from the dromedary camel (Camelus dromedarius) JO: Mol Immunol http://www.kidney.de/pget.php?&tw=1&pmid=32014632 #FOAvip Investigations into the molecular immune response of dromedary camel, a key livestock species of the arid, have been limited due to the lack of species-specific reagents. Here we describe for the first time, the identification and characterization of type I IFNs of dromedary camel, which are the most important cytokines in the innate host immune response against viruses. We cloned camel IFN-alpha coding sequences and identified a total of eleven subtypes. The canonical IFN-alpha subtype designated as IFN-alpha1 contained a 555-bp Open Reading Frame encoding a protein of 184 amino acids. Recombinant IFN-alpha1 protein was produced in E. coli and purified from inclusion bodies. Recombinant camel IFN-alpha1 induced the mRNA expression of interferon-stimulated genes (ISGs) in camel kidney cells. The purified protein also showed potent in-vitro antiviral activity against Camelpox Virus in kidney cells. The identified camel IFN-alpha protein and the subtypes will facilitate a better understanding of the host immune response to viral infections in camel and the development of potential antiviral biologicals for zoonotic diseases for which camel act as a reservoir. Twit Text FOAVip Camelid type I interferons: Identification and functional characterization of interferon alpha from the dromedary camel (Camelus dromedarius) ????Mol Immunol http://www.kidney.de/pget.php?&tw=1&pmid=32014632 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=32014632 #FOAvip English Wikipedia <ref>{{Cite pmid|32014632}}</ref> Camelid type I interferons: Identification and functional characterization of interferon alpha from the dromedary camel (Camelus dromedarius) #MMPMID32014632 Premraj A; Aleyas AG; Nautiyal B; Rasool TJ Mol Immunol 2020[Mar]; 119 (ä): 132-143 PMID32014632show ga Investigations into the molecular immune response of dromedary camel, a key livestock species of the arid, have been limited due to the lack of species-specific reagents. Here we describe for the first time, the identification and characterization of type I IFNs of dromedary camel, which are the most important cytokines in the innate host immune response against viruses. We cloned camel IFN-alpha coding sequences and identified a total of eleven subtypes. The canonical IFN-alpha subtype designated as IFN-alpha1 contained a 555-bp Open Reading Frame encoding a protein of 184 amino acids. Recombinant IFN-alpha1 protein was produced in E. coli and purified from inclusion bodies. Recombinant camel IFN-alpha1 induced the mRNA expression of interferon-stimulated genes (ISGs) in camel kidney cells. The purified protein also showed potent in-vitro antiviral activity against Camelpox Virus in kidney cells. The identified camel IFN-alpha protein and the subtypes will facilitate a better understanding of the host immune response to viral infections in camel and the development of potential antiviral biologicals for zoonotic diseases for which camel act as a reservoir. |Animals[MESH] |Antiviral Agents[MESH] |Camelus/genetics/*immunology[MESH] |Cloning, Molecular[MESH] |Escherichia coli[MESH] |Interferon Type I/chemistry/genetics/*immunology/isolation & purification[MESH] |Orthopoxvirus/*immunology[MESH] |Recombinant Proteins/genetics/immunology[MESH]Camelid type I interferons: Identification and functional characteriza(epmc).pdf DeepDyve Pubget Overpricing