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10.2807/1560-7917.ES.2020.25.3.2000045 http://scihub22266oqcxt.onion/10.2807/1560-7917.ES.2020.25.3.2000045 31992387!6988269!31992387     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Euro+Surveill 2020 ; 25 (3): ä Nephropedia Template TP {{tp|p=31992387|t=2020. Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR |pdf=|usr=}}{{31992387}} gab.com Text Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR JO: Euro Surveill http://www.kidney.de/pget.php?&tw=1&pmid=31992387 #FOAvip BACKGROUND: The ongoing outbreak of the recently emerged novel coronavirus (2019-nCoV) poses a challenge for public health laboratories as virus isolates are unavailable while there is growing evidence that the outbreak is more widespread than initially thought, and international spread through travellers does already occur. AIM: We aimed to develop and deploy robust diagnostic methodology for use in public health laboratory settings without having virus material available. METHODS: Here we present a validated diagnostic workflow for 2019-nCoV, its design relying on close genetic relatedness of 2019-nCoV with SARS coronavirus, making use of synthetic nucleic acid technology. RESULTS: The workflow reliably detects 2019-nCoV, and further discriminates 2019-nCoV from SARS-CoV. Through coordination between academic and public laboratories, we confirmed assay exclusivity based on 297 original clinical specimens containing a full spectrum of human respiratory viruses. Control material is made available through European Virus Archive - Global (EVAg), a European Union infrastructure project. CONCLUSION: The present study demonstrates the enormous response capacity achieved through coordination of academic and public laboratories in national and European research networks. Twit Text FOAVip Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR ????Euro Surveill http://www.kidney.de/pget.php?&tw=1&pmid=31992387 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=31992387 #FOAvip English Wikipedia <ref>{{Cite pmid|31992387}}</ref> Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR #MMPMID31992387 Corman VM; Landt O; Kaiser M; Molenkamp R; Meijer A; Chu DK; Bleicker T; Brunink S; Schneider J; Schmidt ML; Mulders DG; Haagmans BL; van der Veer B; van den Brink S; Wijsman L; Goderski G; Romette JL; Ellis J; Zambon M; Peiris M; Goossens H; Reusken C; Koopmans MP; Drosten C Euro Surveill 2020[Jan]; 25 (3): ä PMID31992387show ga BACKGROUND: The ongoing outbreak of the recently emerged novel coronavirus (2019-nCoV) poses a challenge for public health laboratories as virus isolates are unavailable while there is growing evidence that the outbreak is more widespread than initially thought, and international spread through travellers does already occur. AIM: We aimed to develop and deploy robust diagnostic methodology for use in public health laboratory settings without having virus material available. METHODS: Here we present a validated diagnostic workflow for 2019-nCoV, its design relying on close genetic relatedness of 2019-nCoV with SARS coronavirus, making use of synthetic nucleic acid technology. RESULTS: The workflow reliably detects 2019-nCoV, and further discriminates 2019-nCoV from SARS-CoV. Through coordination between academic and public laboratories, we confirmed assay exclusivity based on 297 original clinical specimens containing a full spectrum of human respiratory viruses. Control material is made available through European Virus Archive - Global (EVAg), a European Union infrastructure project. CONCLUSION: The present study demonstrates the enormous response capacity achieved through coordination of academic and public laboratories in national and European research networks. |*Clinical Laboratory Techniques/methods[MESH] |COVID-19 Testing[MESH] |COVID-19 Vaccines[MESH] |Coronavirus Infections/*diagnosis/*virology[MESH] |Coronavirus/*classification/*genetics/isolation & purification[MESH] |Disease Outbreaks[MESH] |Humans[MESH] |RNA, Viral/analysis[MESH] |Real-Time Polymerase Chain Reaction/methods[MESH]Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR (epmc).pdf DeepDyve Pubget Overpricing