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10.1111/vox.12888 http://scihub22266oqcxt.onion/10.1111/vox.12888 31930543!7169309!31930543     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=31930543&cmd=llinks): Failed to open stream: HTTP request failed! 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Inactivation of three emerging viruses - severe acute respiratory syndrome coronavirus, Crimean-Congo haemorrhagic fever virus and Nipah virus - in platelet concentrates by ultraviolet C light and in plasma by methylene blue plus visible light |pdf=|usr=}}{{31930543}} gab.com Text Inactivation of three emerging viruses - severe acute respiratory syndrome coronavirus, Crimean-Congo haemorrhagic fever virus and Nipah virus - in platelet concentrates by ultraviolet C light and in plasma by methylene blue plus visible light JO: Vox Sang http://www.kidney.de/pget.php?&tw=1&pmid=31930543 #FOAvip BACKGROUND: Emerging viruses like severe acute respiratory syndrome coronavirus (SARS-CoV), Crimean-Congo haemorrhagic fever virus (CCHFV) and Nipah virus (NiV) have been identified to pose a potential threat to transfusion safety. In this study, the ability of the THERAFLEX UV-Platelets and THERAFLEX MB-Plasma pathogen inactivation systems to inactivate these viruses in platelet concentrates and plasma, respectively, was investigated. MATERIALS AND METHODS: Blood products were spiked with SARS-CoV, CCHFV or NiV, and then treated with increasing doses of UVC light (THERAFLEX UV-Platelets) or with methylene blue (MB) plus increasing doses of visible light (MB/light; THERAFLEX MB-Plasma). Samples were taken before and after treatment with each illumination dose and tested for residual infectivity. RESULTS: Treatment with half to three-fourths of the full UVC dose (0.2 J/cm(2) ) reduced the infectivity of SARS-CoV (>/=3.4 log), CCHFV (>/=2.2 log) and NiV (>/=4.3 log) to the limit of detection (LOD) in platelet concentrates, and treatment with MB and a fourth of the full light dose (120 J/cm(2) ) decreased that of SARS-CoV (>/=3.1 log), CCHFV (>/=3.2 log) and NiV (>/=2.7 log) to the LOD in plasma. CONCLUSION: Our study demonstrates that both THERAFLEX UV-Platelets (UVC) and THERAFLEX MB-Plasma (MB/light) effectively reduce the infectivity of SARS-CoV, CCHFV and NiV in platelet concentrates and plasma, respectively. Twit Text FOAVip Inactivation of three emerging viruses - severe acute respiratory syndrome coronavirus, Crimean-Congo haemorrhagic fever virus and Nipah virus - in platelet concentrates by ultraviolet C light and in plasma by methylene blue plus visible light ????Vox Sang http://www.kidney.de/pget.php?&tw=1&pmid=31930543 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=31930543 #FOAvip English Wikipedia <ref>{{Cite pmid|31930543}}</ref> Inactivation of three emerging viruses - severe acute respiratory syndrome coronavirus, Crimean-Congo haemorrhagic fever virus and Nipah virus - in platelet concentrates by ultraviolet C light and in plasma by methylene blue plus visible light #MMPMID31930543 Eickmann M; Gravemann U; Handke W; Tolksdorf F; Reichenberg S; Muller TH; Seltsam A Vox Sang 2020[Apr]; 115 (3): 146-151 PMID31930543show ga BACKGROUND: Emerging viruses like severe acute respiratory syndrome coronavirus (SARS-CoV), Crimean-Congo haemorrhagic fever virus (CCHFV) and Nipah virus (NiV) have been identified to pose a potential threat to transfusion safety. In this study, the ability of the THERAFLEX UV-Platelets and THERAFLEX MB-Plasma pathogen inactivation systems to inactivate these viruses in platelet concentrates and plasma, respectively, was investigated. MATERIALS AND METHODS: Blood products were spiked with SARS-CoV, CCHFV or NiV, and then treated with increasing doses of UVC light (THERAFLEX UV-Platelets) or with methylene blue (MB) plus increasing doses of visible light (MB/light; THERAFLEX MB-Plasma). Samples were taken before and after treatment with each illumination dose and tested for residual infectivity. RESULTS: Treatment with half to three-fourths of the full UVC dose (0.2 J/cm(2) ) reduced the infectivity of SARS-CoV (>/=3.4 log), CCHFV (>/=2.2 log) and NiV (>/=4.3 log) to the limit of detection (LOD) in platelet concentrates, and treatment with MB and a fourth of the full light dose (120 J/cm(2) ) decreased that of SARS-CoV (>/=3.1 log), CCHFV (>/=3.2 log) and NiV (>/=2.7 log) to the LOD in plasma. CONCLUSION: Our study demonstrates that both THERAFLEX UV-Platelets (UVC) and THERAFLEX MB-Plasma (MB/light) effectively reduce the infectivity of SARS-CoV, CCHFV and NiV in platelet concentrates and plasma, respectively. |*Light[MESH] |*Ultraviolet Rays[MESH] |*Virus Inactivation[MESH] |Blood Platelets/virology[MESH] |Blood Transfusion[MESH] |Hemorrhagic Fever Virus, Crimean-Congo/drug effects/*radiation effects[MESH] |Humans[MESH] |Methylene Blue/*pharmacology[MESH] |Nipah Virus/drug effects/*radiation effects[MESH] |Plasma/virology[MESH]Inactivation of three emerging viruses - severe acute respiratory synd(epmc).pdf DeepDyve Pubget Overpricing