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10.1007/978-1-0716-0211-9_11 http://scihub22266oqcxt.onion/10.1007/978-1-0716-0211-9_11 31883093!7122216!31883093     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Methods+Mol+Biol 2020 ; 2099 (ä): 127-133 Nephropedia Template TP {{tp|p=31883093|t=2020. Qualitative and Quantitative Determination of MERS-CoV S1-Specific Antibodies Using ELISA |pdf=|usr=}}{{31883093}} gab.com Text Qualitative and Quantitative Determination of MERS-CoV S1-Specific Antibodies Using ELISA JO: Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=31883093 #FOAvip Indirect enzyme-linked immunosorbent assay (ELISA) enables detection and quantification of antigen-specific antibodies in biological samples such as human or animal sera. Most current MERS-CoV serological assays such as neutralization, immunofluorescence, or protein microarray rely on handling of live MERS-CoV in high containment laboratories, highly trained personnel as well as the need for expensive and special equipment and reagents representing a hurdle for most laboratories especially when resources are limited. In this chapter, we describe a validated and optimized indirect ELISA protocol based on recombinant S1 subunit (amino acids 1-725) of MERS-CoV for qualitative and quantitative determination of MERS-CoV-binding antibodies. Twit Text FOAVip Qualitative and Quantitative Determination of MERS-CoV S1-Specific Antibodies Using ELISA ????Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=31883093 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=31883093 #FOAvip English Wikipedia <ref>{{Cite pmid|31883093}}</ref> Qualitative and Quantitative Determination of MERS-CoV S1-Specific Antibodies Using ELISA #MMPMID31883093 Al-Amri SS; Hashem AM Methods Mol Biol 2020[]; 2099 (ä): 127-133 PMID31883093show ga Indirect enzyme-linked immunosorbent assay (ELISA) enables detection and quantification of antigen-specific antibodies in biological samples such as human or animal sera. Most current MERS-CoV serological assays such as neutralization, immunofluorescence, or protein microarray rely on handling of live MERS-CoV in high containment laboratories, highly trained personnel as well as the need for expensive and special equipment and reagents representing a hurdle for most laboratories especially when resources are limited. In this chapter, we describe a validated and optimized indirect ELISA protocol based on recombinant S1 subunit (amino acids 1-725) of MERS-CoV for qualitative and quantitative determination of MERS-CoV-binding antibodies. |Animals[MESH] |Antibodies, Monoclonal/*immunology[MESH] |Antibodies, Neutralizing/*immunology[MESH] |Antibodies, Viral/immunology[MESH] |Coronavirus Infections/*virology[MESH] |Enzyme-Linked Immunosorbent Assay/*methods[MESH] |Humans[MESH] |Middle East Respiratory Syndrome Coronavirus/*immunology/isolation & purification[MESH] |Recombinant Proteins[MESH]Qualitative and Quantitative Determination of MERS-CoV S1-Specific Ant(epmc).pdf DeepDyve Pubget Overpricing