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10.1007/978-1-0716-0211-9_10 http://scihub22266oqcxt.onion/10.1007/978-1-0716-0211-9_10 31883092!7123069!31883092     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=31883092&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 245.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Methods+Mol+Biol 2020 ; 2099 (ä): 117-126 Nephropedia Template TP {{tp|p=31883092|t=2020. Generation of MERS-CoV Pseudotyped Viral Particles for the Evaluation of Neutralizing Antibodies in Mammalian Sera |pdf=|usr=}}{{31883092}} gab.com Text Generation of MERS-CoV Pseudotyped Viral Particles for the Evaluation of Neutralizing Antibodies in Mammalian Sera JO: Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=31883092 #FOAvip Pseudotyped viral particle production has been used extensively and broadly for many viruses to evaluate levels of neutralizing antibodies, viral entry inhibitors and vaccine immunogenicity. This assay is extremely safe and useful alternative to live virus-based assay without the need for high containment facilities. In this chapter, we describe the generation of MERS-CoV pseudotyped viral particles (MERSpp) expressing full-length spike protein using second-generation lentiviral packaging system. This platform is optimized to generate high titer of MERSpp and to test sera from different mammalian species. Twit Text FOAVip Generation of MERS-CoV Pseudotyped Viral Particles for the Evaluation of Neutralizing Antibodies in Mammalian Sera ????Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=31883092 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=31883092 #FOAvip English Wikipedia <ref>{{Cite pmid|31883092}}</ref> Generation of MERS-CoV Pseudotyped Viral Particles for the Evaluation of Neutralizing Antibodies in Mammalian Sera #MMPMID31883092 Almasaud A; Alharbi NK; Hashem AM Methods Mol Biol 2020[]; 2099 (ä): 117-126 PMID31883092show ga Pseudotyped viral particle production has been used extensively and broadly for many viruses to evaluate levels of neutralizing antibodies, viral entry inhibitors and vaccine immunogenicity. This assay is extremely safe and useful alternative to live virus-based assay without the need for high containment facilities. In this chapter, we describe the generation of MERS-CoV pseudotyped viral particles (MERSpp) expressing full-length spike protein using second-generation lentiviral packaging system. This platform is optimized to generate high titer of MERSpp and to test sera from different mammalian species. |Animals[MESH] |Antibodies, Neutralizing/*blood[MESH] |Coronavirus Infections/*virology[MESH] |HEK293 Cells[MESH] |Humans[MESH] |Immunogenicity, Vaccine/immunology[MESH] |Mammals[MESH] |Middle East Respiratory Syndrome Coronavirus/*immunology/isolation & purification[MESH] |Neutralization Tests[MESH] |Spike Glycoprotein, Coronavirus/genetics/*immunology/metabolism[MESH] |Viral Vaccines/*immunology[MESH] |Virion/genetics/*immunology/metabolism[MESH]Generation of MERS-CoV Pseudotyped Viral Particles for the Evaluation (epmc).pdf DeepDyve Pubget Overpricing