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10.1007/978-1-0716-0211-9_9 http://scihub22266oqcxt.onion/10.1007/978-1-0716-0211-9_9 31883091!7121888!31883091     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=31883091&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Methods+Mol+Biol 2020 ; 2099 (ä): 107-116 Nephropedia Template TP {{tp|p=31883091|t=2020. Evaluation of MERS-CoV Neutralizing Antibodies in Sera Using Live Virus Microneutralization Assay |pdf=|usr=}}{{31883091}} gab.com Text Evaluation of MERS-CoV Neutralizing Antibodies in Sera Using Live Virus Microneutralization Assay JO: Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=31883091 #FOAvip The microneutralization (MN) assay is a standard and important technique in virology, immunology, and epidemiology. It is a highly specific and sensitive assay for evaluating virus-specific neutralizing antibodies (nAbs) in human and animal sera. It provides the most precise answer to whether or not an individual or animal has antibodies that can neutralize or inhibit the infectivity of a specific virus strain. However, using live virus-based MN assay might require working under high containment facilities especially when dealing with high-risk pathogens such as the Middle East respiratory syndrome-coronavirus (MERS-CoV). In this chapter, we describe the isolation, amplification, and titration of MERS-CoV, as well as detailed MN assay to measure nAb levels in sera from different mammalian species. Twit Text FOAVip Evaluation of MERS-CoV Neutralizing Antibodies in Sera Using Live Virus Microneutralization Assay ????Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=31883091 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=31883091 #FOAvip English Wikipedia <ref>{{Cite pmid|31883091}}</ref> Evaluation of MERS-CoV Neutralizing Antibodies in Sera Using Live Virus Microneutralization Assay #MMPMID31883091 Algaissi A; Hashem AM Methods Mol Biol 2020[]; 2099 (ä): 107-116 PMID31883091show ga The microneutralization (MN) assay is a standard and important technique in virology, immunology, and epidemiology. It is a highly specific and sensitive assay for evaluating virus-specific neutralizing antibodies (nAbs) in human and animal sera. It provides the most precise answer to whether or not an individual or animal has antibodies that can neutralize or inhibit the infectivity of a specific virus strain. However, using live virus-based MN assay might require working under high containment facilities especially when dealing with high-risk pathogens such as the Middle East respiratory syndrome-coronavirus (MERS-CoV). In this chapter, we describe the isolation, amplification, and titration of MERS-CoV, as well as detailed MN assay to measure nAb levels in sera from different mammalian species. |Animals[MESH] |Antibodies, Neutralizing/*blood[MESH] |Chlorocebus aethiops[MESH] |Coronavirus Infections/*virology[MESH] |Cytopathogenic Effect, Viral[MESH] |Humans[MESH] |Mammals[MESH] |Middle East Respiratory Syndrome Coronavirus/*immunology[MESH] |Neutralization Tests[MESH]Evaluation of MERS-CoV Neutralizing Antibodies in Sera Using Live Viru(epmc).pdf DeepDyve Pubget Overpricing