Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1007/978-1-0716-0211-9_6 http://scihub22266oqcxt.onion/10.1007/978-1-0716-0211-9_6 31883088!7120962!31883088     free     free     free Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\31883088.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Methods+Mol+Biol 2020 ; 2099 (ä): 69-85 Nephropedia Template TP {{tp|p=31883088|t=2020. Deducing the Crystal Structure of MERS-CoV Helicase |pdf=|usr=}}{{31883088}} gab.com Text Deducing the Crystal Structure of MERS-CoV Helicase JO: Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=31883088 #FOAvip RNA virus encodes a helicase essential for viral RNA transcription and replication when the genome size is larger than 7 kb. Coronavirus (CoV) has an exceptionally large RNA genome (~30 kb) and it encodes an essential replicase, the nonstructural protein 13 (nsp13), a member of superfamily 1 helicases. Nsp13 is among the evolutionary most conserved proteins not only in CoVs but also in nidovirales. Thus, it is considered as an important drug target. However, the high-resolution structure of CoV nsp13 remained unavailable even until more than a decade after the outbreak of the severe acute respiratory syndrome coronavirus (SARS-CoV) in 2003, which hindered the structure-based drug design. This is in part due to the intrinsic flexibility of nsp13. Here, we describe protocols of deducing the crystal structure of Middle East respiratory syndrome coronavirus (MERS-CoV) helicase in detail, which include protein expression, purification, crystallization, enzymatic characterization, and structure determination. With these methods, catalytically active recombinant MERS-CoV nsp13 protein can be prepared and crystallized and the crystal structure can be solved. Twit Text FOAVip Deducing the Crystal Structure of MERS-CoV Helicase ????Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=31883088 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=31883088 #FOAvip English Wikipedia <ref>{{Cite pmid|31883088}}</ref> Deducing the Crystal Structure of MERS-CoV Helicase #MMPMID31883088 Cui S; Hao W Methods Mol Biol 2020[]; 2099 (ä): 69-85 PMID31883088show ga RNA virus encodes a helicase essential for viral RNA transcription and replication when the genome size is larger than 7 kb. Coronavirus (CoV) has an exceptionally large RNA genome (~30 kb) and it encodes an essential replicase, the nonstructural protein 13 (nsp13), a member of superfamily 1 helicases. Nsp13 is among the evolutionary most conserved proteins not only in CoVs but also in nidovirales. Thus, it is considered as an important drug target. However, the high-resolution structure of CoV nsp13 remained unavailable even until more than a decade after the outbreak of the severe acute respiratory syndrome coronavirus (SARS-CoV) in 2003, which hindered the structure-based drug design. This is in part due to the intrinsic flexibility of nsp13. Here, we describe protocols of deducing the crystal structure of Middle East respiratory syndrome coronavirus (MERS-CoV) helicase in detail, which include protein expression, purification, crystallization, enzymatic characterization, and structure determination. With these methods, catalytically active recombinant MERS-CoV nsp13 protein can be prepared and crystallized and the crystal structure can be solved. |Coronavirus Infections/*virology[MESH] |Crystallization[MESH] |Humans[MESH] |Methyltransferases/*chemistry[MESH] |Middle East Respiratory Syndrome Coronavirus/*enzymology[MESH] |Models, Molecular[MESH] |RNA Helicases/*chemistry[MESH] |RNA, Viral/genetics[MESH] |Viral Nonstructural Proteins/*chemistry[MESH]Deducing the Crystal Structure of MERS-CoV Helicase (epmc).pdf DeepDyve Pubget Overpricing