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Crystallization and Structural Determination of the Receptor-Binding Domain of MERS-CoV Spike Glycoprotein #MMPMID31883086
Zhou H; Zhang S; Wang X
Methods Mol Biol 2020[]; 2099 (ä): 39-50 PMID31883086show ga
Three-dimensional structures of the receptor-binding domain (RBD) of MERS-CoV spike glycoprotein bound to cellular receptor and monoclonal antibodies (mAbs) have been determined by X-ray crystallography, providing structural information about receptor recognition and neutralizing mechanisms of mAbs at the atomic level. In this chapter, we describe the purification, crystallization, and structure determination of the MERS-CoV RBD.
|Coronavirus Infections/*virology[MESH]
|Crystallization[MESH]
|Crystallography, X-Ray/*methods[MESH]
|Humans[MESH]
|Middle East Respiratory Syndrome Coronavirus/*metabolism[MESH]