Methods Mol Biol 2020[]; 2099 (ä): 9-20 PMID31883084show ga
Middle East respiratory syndrome coronavirus (MERS-CoV) is an emerging zoonotic pathogen with a broad host range. The extent of MERS-CoV in nature can be traced to its adaptable cell entry steps. The virus can bind host-cell carbohydrates as well as proteinaceous receptors. Following receptor interaction, the virus can utilize diverse host proteases for cleavage activation of virus-host cell membrane fusion and subsequent genome delivery. The fusion and genome delivery steps can be completed at variable times and places, either at or near cell surfaces or deep within endosomes. Investigators focusing on the CoVs have developed several methodologies that effectively distinguish these different cell entry pathways. Here we describe these methods, highlighting virus-cell entry factors, entry inhibitors, and viral determinants that specify the cell entry routes. While the specific methods described herein were utilized to reveal MERS-CoV entry pathways, they are equally suited for other CoVs, as well as other protease-dependent viral species.
|*Virus Internalization[MESH]
|Cell Membrane/virology[MESH]
|Coronavirus Infections/*virology[MESH]
|Endosomes/virology[MESH]
|Genome, Viral/*genetics[MESH]
|HEK293 Cells[MESH]
|Humans[MESH]
|Membrane Proteins/metabolism[MESH]
|Middle East Respiratory Syndrome Coronavirus/genetics/isolation & purification/*physiology[MESH]
free
free
free
Methods+Mol+Biol 2020 ; 2099 (ä): 9-20
