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  • Studying Evolutionary Adaptation of MERS-CoV #MMPMID31883083
  • Letko M; Munster V
  • Methods Mol Biol 2020[]; 2099 (ä): 3-8 PMID31883083show ga
  • Forced viral adaptation is a powerful technique employed to study the ways viruses may overcome various selective pressures that reduce viral replication. Here, we describe methods for in vitro serial passaging of Middle East respiratory syndrome coronavirus (MERS-CoV) to select for mutations which increase replication on semi-permissive cell lines as described in Letko et al., Cell Rep 24, 1730-1737, 2018.
  • |Adaptation, Physiological/*genetics[MESH]
  • |Animals[MESH]
  • |Biological Evolution[MESH]
  • |Cell Line[MESH]
  • |Chlorocebus aethiops[MESH]
  • |Coronavirus Infections/*virology[MESH]
  • |Host Specificity[MESH]
  • |Humans[MESH]
  • |Middle East Respiratory Syndrome Coronavirus/*genetics/physiology[MESH]
  • |Serial Passage[MESH]
  • |Vero Cells[MESH]
  • |Virus Replication/*genetics[MESH]

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  • suck abstract from ncbi

    3 ä.2099 2020