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10.1016/j.antiviral.2019.104697 http://scihub22266oqcxt.onion/10.1016/j.antiviral.2019.104697 31863793!7114316!31863793     free     free     free Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 253.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Antiviral+Res 2020 ; 174 (ä): 104697 Nephropedia Template TP {{tp|p=31863793|t=2020. In silico and in vitro analysis of small molecules and natural compounds targeting the 3CL protease of feline infectious peritonitis virus |pdf=|usr=}}{{31863793}} gab.com Text In silico and in vitro analysis of small molecules and natural compounds targeting the 3CL protease of feline infectious peritonitis virus JO: Antiviral Res http://www.kidney.de/pget.php?&tw=1&pmid=31863793 #FOAvip The computational search of chemical libraries has been used as a powerful tool for the rapid discovery of candidate compounds. To find small molecules with anti-feline infectious peritonitis virus (FIPV) properties, we utilized a virtual screening technique to identify the active site on the viral protease for the binding of the available natural compounds. The protease 3CL (3CL(pro)) plays an important role in the replication cycle of FIPV and other viruses within the family Coronaviridae. The 15 best-ranked candidate consensus compounds, based on three docking tools, were evaluated for further assays. The protease inhibitor assay on recombinant FIPV 3CL(pro) was performed to screen the inhibitory effect of the candidate compounds with IC(50) ranging from 6.36 +/- 2.15 to 78.40 +/- 2.60 muM. As determined by the cell-based assay, the compounds NSC345647, NSC87511, and NSC343256 showed better EC(50) values than the broad-spectrum antiviral drug ribavirin and the protease inhibitor lopinavir, under all the test conditions including pre-viral entry, post-viral entry, and prophylactic activity. The NSC87511 particularly yielded the best selective index (>4; range of SI = 13.80-22.90). These results indicated that the natural small-molecular compounds specifically targeted the 3CL(pro) of FIPV and inhibited its replication. Structural modification of these compounds may generate a higher anti-viral potency for the further development of a novel therapy against FIP. Twit Text FOAVip In silico and in vitro analysis of small molecules and natural compounds targeting the 3CL protease of feline infectious peritonitis virus ????Antiviral Res http://www.kidney.de/pget.php?&tw=1&pmid=31863793 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=31863793 #FOAvip English Wikipedia <ref>{{Cite pmid|31863793}}</ref> In silico and in vitro analysis of small molecules and natural compounds targeting the 3CL protease of feline infectious peritonitis virus #MMPMID31863793 Theerawatanasirikul S; Kuo CJ; Phetcharat N; Lekcharoensuk P Antiviral Res 2020[Feb]; 174 (ä): 104697 PMID31863793show ga The computational search of chemical libraries has been used as a powerful tool for the rapid discovery of candidate compounds. To find small molecules with anti-feline infectious peritonitis virus (FIPV) properties, we utilized a virtual screening technique to identify the active site on the viral protease for the binding of the available natural compounds. The protease 3CL (3CL(pro)) plays an important role in the replication cycle of FIPV and other viruses within the family Coronaviridae. The 15 best-ranked candidate consensus compounds, based on three docking tools, were evaluated for further assays. The protease inhibitor assay on recombinant FIPV 3CL(pro) was performed to screen the inhibitory effect of the candidate compounds with IC(50) ranging from 6.36 +/- 2.15 to 78.40 +/- 2.60 muM. As determined by the cell-based assay, the compounds NSC345647, NSC87511, and NSC343256 showed better EC(50) values than the broad-spectrum antiviral drug ribavirin and the protease inhibitor lopinavir, under all the test conditions including pre-viral entry, post-viral entry, and prophylactic activity. The NSC87511 particularly yielded the best selective index (>4; range of SI = 13.80-22.90). These results indicated that the natural small-molecular compounds specifically targeted the 3CL(pro) of FIPV and inhibited its replication. Structural modification of these compounds may generate a higher anti-viral potency for the further development of a novel therapy against FIP. |Animals[MESH] |Antiviral Agents/*chemistry/pharmacology[MESH] |Catalytic Domain[MESH] |Cats[MESH] |Computer Simulation[MESH] |Coronavirus, Feline/chemistry/drug effects/*enzymology/genetics[MESH] |Drug Evaluation, Preclinical[MESH] |Feline Infectious Peritonitis/*virology[MESH] |Kinetics[MESH] |Peptide Hydrolases/*chemistry/genetics/metabolism[MESH] |Protease Inhibitors/*chemistry/pharmacology[MESH] |Ribavirin/chemistry/pharmacology[MESH]In silico and in vitro analysis of small molecules and natural compoun(epmc).pdf DeepDyve Pubget Overpricing